The embodiment of the invention discloses an OLED array substrate, a manufacturing method of the OLED array substrate and a display device with the OLED array substrate, and belongs to the technical field of display. The OLED array substrate, the manufacturing method of the OLED array substrate and the display device with the OLED array substrate solve the technical problems that a TFT of an existing active matrix type OLED display device is easily illuminated by light, and the display effect is influenced. The OLED array substrate comprises thin film transistors arranged on a substrate, wherein black matrixes are arranged on the thin film transistors, a through hole is formed in each black matrix, first electrodes, a luminous layer and a second electrode are arranged on the tops of the black matrixes in sequence from bottom to top, the first electrodes are connected with the thin film transistors through the through holes, and every two adjacent first electrodes are separated through a blocking wall. The OLED array substrate can be applied to display devices such as OLED panels, television sets, displayers, digital photo frames, mobile phones and tablet personal computers.