The invention discloses a method for preparing recombinant collagen
antioxidant microspheres without a cross-linking agent. The method comprises the following steps: 1, dissolving recombinant collagen and
chitosan in deionized water containing glacial
acetic acid to serve as a water phase;
petroleum ether containing a surfactant is used as an
oil phase; 2, injecting the
oil phase into the water phase, emulsifying and stirring through a
homogenizer, and reducing the temperature of the
system to obtain an
emulsion; 3, a fixing agent, water and absolute ethyl
alcohol are blended and precooled, and fixing liquid is obtained; 4, adding the stationary liquid into the emulsified liquid for curing, and washing the cured microspheres; and 5, transferring into a
tannic acid aqueous solution to soak, then washing with deionized water, and freeze-
drying to obtain the recombinant collagen microspheres with
uniform size. The recombinant collagen microspheres prepared by the method have good
dispersity and uniform and controllable size, avoid the
toxicity problem of a cross-linking agent, can be applied to the fields of
medical instruments,
tissue engineering,
drug delivery and the like as a filling agent, and have wide commercial application prospects.