The invention provides a production method of an HRB500E high-strength and anti-seismic rebar. The production method comprises the step that a billet is manufactured. The billet is prepared from the components in percentage: 0.20%-0.25% of C, 0.40%-0.70% of Si, 1.30%-1.60% of Mn, less than or equal to 0.045% of P, less than or equal to 0.045% of S, 0.020%-0.050% of V, 0.0050%-0.0120% of N, and thebalance of Fe and inevitable impurities; the components are basically the same as component formulas used in the production of HRB400 and HRB400E, steel has multiple stages, namely, by adjusting rolling processes, the production of HRB500E and HRB400E can be realized with the same chemical composition formula. Therefore, according to the production method of the HRB500E high-strength and anti-seismic rebar, a unified production technique can be adopted in the preparation of raw materials and blank, the production efficiency is improved favourably, the frequencies of adjusting preparation parameters such as temperature and the proportion of the components are reduced, the stability of products is improved, and the production efficiency is improved favourably.