The invention discloses an in-line fuse capable of achieving safety on-line detection. The in-line fuse comprises a shell, detonator modules, a high-voltage initiation module, a control circuit module, an electrical interface and a testing interface, wherein the detonator modules are installed on the left side and the right side of the bottom of the shell respectively, and the electrical interfaceand the testing interface are inserted on the left side and the right side of the top of the shell respectively; the left side and the right side of the interior of the shell are each provided with astand column, a high-voltage initiation module and a control circuit module are sequentially arranged between the stand columns from bottom to top, and the left end and the right end of the high-voltage initiation module and the left end and the right end of the control circuit module are both connected with the stand columns through positioning mechanisms; and one end of each detonator module, one end of the high-voltage initiation module, one end of the control circuit module, one end of the electrical interface and one end of the testing interface are each provided with a wiring mechanism.According to the in-line fuse capable of achieving safety on-line detection, on-line high-efficiency detection of the in-line fuses is realized, the assembly efficiency of the in-line fuses is improved, and the safety during use of the in-line fuses is improved.