The invention relates to a method for recovering acetylisovaleryltylosin tartrate from an acetylisovaleryltylosin tartrate crystallization mother liquor. The method comprises the following steps: firstly, adding a surfactant into the acetylisovaleryltylosin tartrate crystallization mother liquor, then concentrating a thin film by generating air bubbles, collecting air bubble films, cooling the collected air bubble films, adding a separating agent, separating, filtering, crystallizing the obtained filtrate, and washing with water to obtain pure acetylisovaleryltylosin tartrate. The acetylisovaleryltylosin tartrate is recovered by adopting a simple method, so that the problems that the acetylisovaleryltylosin tartrate crystallization mother liquor is too low in unit content of acetylisovaleryltylosin tartrate and acetylisovaleryltylosin tartrate is poor in thermal stability and can not be recovered can be solved, the recycling of acetylisovaleryltylosin tartrate can be realized, the available resources can be increased, the cost can be lowered, the recovery rate of the finished product can be enhanced, the content of pollutants in discharged wastewater can be reduced, the pollution to environment can be alleviated, and certain social and economical benefits can be achieved.