The invention discloses a nondestructive storage method of a
fluorescence spectrum binary concentration
image sequence based on multichannel bit mapping and entropy coding. Firstly, N frames of binary edge images with the same resolution are fused bit by bit according to pixels to form a fusion matrix V; and determining the storage bit width of the V, carrying out
run length coding on the V, constructing a Huffman
code table based on the statistical probability, carrying out entropy compression, and finally generating a compressed file. The header of the file records information such as the image size, the frame number, the
data type and the CRC check code, and the integrity and the decompressibility of compressed data are ensured. And during decompression, performing
Huffman decoding, inverse RLE and bitwise disassembly according to an inverted sequence to realize complete lossless
recovery of an original sequence. By using the method, redundancy among multiple frames of images can be fully utilized, the
compression ratio is improved, lossless reconstruction of
original data is realized, and the method is particularly suitable for a
binary image sequence scene with strong inter-frame correlation. In a multi-frame related scene, the
compression ratio of the method is obviously superior to that of a traditional frame-by-frame PNG method, and the
reproduction degree reaches 100%.