The invention discloses a method and device for testing Ethernet real-time service quality on the basis of point-and-point testing, and belongs to the field of Ethernet performance testing. According to the method, testing data packets with timestamp values are sent back and forth between a master device and a slave device, so that measurement of a one-way delay value and one-way delay jitter is achieved, and synchronous operation is accomplished; according to the device, the master device and the slave device are connected through an intermediate network, the master device is used for generating the testing data packets and leading the testing process, the salve device is used for receiving the data packets, and the testing process of the slave device is controlled by the master device. According to the method and device, due to the fact that delay measurement of the master device and delay measurement of the slave device are carried out according to relative clock adjustment of the master device and relative clock adjustment of the slave device respectively, the synchronizing process of the testing time can be accomplished in two steps, a whole network synchronous clock is not needed, special processing of a middle node device is not needed, the testing environment is simplified, precision, achieved by hardware, of a local timer can be at the microsecond level, and the testing process does not have any influence on the normal service.