The invention relates to a large aluminum piece milling and positioning clamp which comprises a plurality of first positioning blocks, a plurality of second positioning blocks and a plurality of third positioning blocks. The first positioning blocks, the second positioning blocks and the third positioning blocks are arranged side by side and provided with first steps, wherein the first steps are provided with kidney-shaped holes. The first positioning blocks and the second positioning blocks are provided with second steps, wherein ejection rods are installed on the second steps, and the bottom ends of the ejection rods are connected with a handle. The second steps of the first positioning blocks are provided with stopping blocks. The third positioning blocks consist of L-shaped positioning plates and flat plates which are connected fixedly, wherein the positioning plates are provided with adjusting bolts, and the flat plates are provided with flat plates. The large aluminum piece milling and positioning clamp further comprises a positioning part, wherein the positioning part is provided with a locking bolt. The large aluminum piece milling and positioning clamp is simple in structure, flexible and convenient to use and high in clamping efficiency, and the accuracy is easy to ensure.