The invention discloses an open-cut type non-column large-span arched subway station structure as well as a design and construction method and relates to the field of subway and underground engineering as well as underground pipe gallery construction. The structure comprises a bottom plate, side walls, middle plates, middle beams, arched plates, arched beams, reinforcing tips and prestressed cables; the two side walls in parallel are oppositely arranged on the bottom plate; the multiple arched beams are evenly arranged in the passage direction; one arched plate is arranged between every two arched beams; the arched plates located on two sides of the same arched beam are connected with each other; one prestressed cable is arranged between two arch feet of the same arched beam; the middle beams are arranged in the middles of the two side walls; and one middle plate is arranged between the every two adjacent middle beams. The method is as follows: the prestressed cables are anchored and tensioned among the bottom plate, the side walls and the middle plates, among the middle beams, the side walls and the arched beams, among the arched plates, among the reinforcing tips and top ends of the side walls. With the structure and the method, the carrying force of end weak areas and the overall rigidity are improved, the lost height of the arched beams is increased, the beam height is reduced, the dead weight is reduced, and the phenomena of concrete cracking on lower edges and crushing on upper edges of the beams are avoided.