The invention discloses a cross slope control method for ultra-wide steel box girder assembly. The cross slope control method comprises the steps that an assembly jig frame is manufactured; a tooth plate is arranged on the tread; the elevation value of the inclined tread upper tooth plate is corrected according to the design reversible deformation of the steel box girder inclined bottom plate; a flat bottom plate of the steel box girder is placed on the tooth plates on the horizontal tread according to the designed elevation, inclined bottom plates on the two sides of the steel box girder are placed on the corrected tooth plates, and the flat bottom plate and the inclined bottom plates on the two sides are assembled, positioned, welded and fixed; then, the web and the partition plate are assembled, positioned, welded and fixed, vertical butt weld joints of the partition plate are welded firstly, then vertical butt weld joints of the partition plate and the web are welded, and finally transverse structure weld joints of the partition plate and the bottom plate are welded; and finally, the top plate units are assembled, positioned, welded and fixed, butt welding seams of the top plate units are welded firstly, then butt welding seams of the transverse connecting plates on the top plate units are welded, and finally transverse structure welding seams of the transverse connecting plates and the partition plates are welded. According to the method, the structural size precision control level in steel box girder manufacturing is improved, and the cross slope precision of the ultra-wide bridge floor is guaranteed.