The invention discloses a box-building type optical fiber splicing unit capable of being repeatedly opened and used, comprising a front-mounted box assembly used for fixing an optical fiber and a rear-mounted box assembly used for fixing the optical cable coating of the optical fiber, wherein and the front-mounted box assembly and the rear-mounted box assembly are fixedly connected; the front-mounted box assembly comprises a shell, a building cover, a front-mounted box, a V-slot metal block, a lock pin and a guide block, and the building cover is arranged on the shell; the front-mounted box, the V-slot metal block, the lock pin and the guide block are arranged in the shell; the box is provided with floating ribs and building slots; the building cover comprises building arms and building bodies; the building bodies and the building slots fit; the shell is also provided with an opening cover; the opening cover and the building cover are relatively arranged; the opening cover comprises at least one top plate; and the top plate can act on the building arms of the building cover and open the building cover. The optical fiber splicing unit has simple and reliable splicing structure, short site operating time and low overall manufacture cost and greatly reduces the terminating difficulty of optical fiber splicing.