The invention relates to a manufacturing method of polyacetal copolymer. The manufacturing method is capable of reducing scales, and can stably and continuously produce high quality polyacetal copolymer in a long term in a high yield. The manufacturing method comprises the following steps: mixing cyclic ether and/or cyclic condensed formaldehyde, a polymerization catalyst, acetal, which is represented by the formula (1), with a low molecular weight, an organic solvent, and an antioxidant to obtain a pre-mixed solution; and then feeding the pre-mixed solution and trioxane into a polymerization machine to carry out polymerization. Formula (1): R-(CH2-O)n-R.... in the formula (1), the R represents a free hydrogen atom, a straight chain alkyl group or a branched alkyl group, a straight chain alkoxyl group or a branched alkoxyl group, or a hydroxyl group, and n represents an integer in a range of 1 to 20.