The invention discloses wear-resistant conductive shoe sole rubber as well as a preparation method and an application thereof. The wear-resistant conductive shoe sole rubber contains the following components in parts by weight: 50-60 parts of standard rubber, 40-50 parts of butadiene-styrene rubber, 1.8-2.2 parts of sulfur, 0.1-0.3 part of a peptizer DS-T-1, 2.8-3.2 parts of a vulcanizing accelerator, 3.3-3.7 parts of stearic acid, 4.6-5.4 parts of activated zinc oxide, 20-26 parts of rubber softening oil, 8-10 parts of dibutyl phthalate, 65-75 parts of N330 carbon black, 3.8-4.2 parts of an anti-ageing agent, 6-8 parts of modified petroleum resin, 0.4-0.6 part of salicylic acid and 9-11 parts of conductive carbon black. By screening and optimizing a sizing agent formula of the rubber, a product prepared from a sizing agent prepared by virtue of the method has good integrated wear resistance and conductivity, the wear volume of the vulcanized shoe sole rubber is less than 0.3cm<3> / 1.61km, and the resistance value of the shoe sole rubber is (0.1kilo-ohm)-(100kilo-ohm). The preparation method is simple, feasible, wide in raw material source and low in cost, and the use effect of theproduct is good.