The invention discloses a
matrix decomposition-based lensless holographic microscopic
speckle noise removing method and a
matrix decomposition-based lensless holographic microscopic
speckle noise removing device. The
matrix decomposition-based lensless holographic microscopic
speckle noise removing method comprises the following steps: S1, turning off a
light source, and acquiring a dark field image; S2, turning on the
light source, and acquiring a
bright field image under uniform
irradiation of the
light source; S3, placing a particle-containing solution sample above a sensor, guaranteeing that the distance from the sample to the sensor is much smaller than the distance from the sample to the light source, turning on the light source, and acquiring a holographic
image sequence of the sample; S4, performing
flat field correction on any hologram image required to be calculated; S5, performing
noise separation on the corrected holographic image by a matrix
decomposition algorithm, and decomposing the corrected holographic image into two parts, namely a particle hologram part and a
background noise part; S6, further performing
image analysis and
processing on the calculated holographic image. Through the matrix
decomposition-based lensless holographic microscopic speckle
noise removing method and the matrix
decomposition-based lensless holographic microscopic speckle
noise removing device, the speckle noise as well as interference fringe noise generated by multiple reflections of the sample can be removed, so that high-precision dynamic 3D imaging is achieved.