In order to solve a plurality of defects of the aerodynamic characteristic determination of the micro-particles of the preparation by adopting a cascade impactor, the invention provides a test devicefor improving the in-vivo and in-vitro correlation of an inhaled preparation. The device comprises a drug delivery device 1, a human respiratory tract model 2 and a breathing simulator 3, wherein thehuman respiratory tract model 2 comprises an adapter 21 and a real human respiratory tract 3D solid model 22, the breathing simulator 3 comprises a connection control assembly 31, a piston type movement device 32, a control system 33 and a breathing data acquisition device 34, and the device is characterized in that the real human respiratory tract 3D solid model 22 is provided with a plurality offirst quick-connection interfaces 221, the plurality of first quick-connection interfaces 221 are finally converged into a total air path through a plurality of air paths and then connected to the inlet end of the connection control assembly 31, the outlet end of the connection control assembly 32 is connected to the piston type movement device 32; and the connection control assembly 31 comprisesa first solenoid valve 311, a second solenoid valve 312, a third solenoid valve 313, an electronic flow meter 315, and a main body air path.