An extraction method for
antioxidant polypeptide in Guangchang
nymphaea alba is characterized by comprising the following steps of weighting a quantity of
protein of Guangchang
nymphaea alba for preparing water solution with
substrate concentration of 3-6%, heating the solution rapidly to 80-85 DEG C after
dissolution, and keeping the temperature for 10-15 minutes; after pretreatment, adding
phosphate buffer so as to adjust the pH to 7.0-8.5, adding
trypsin, wherein the enzymolysis temperature is 45-60 DEG C, and the
enzyme use amount is 6-9% of
substrate concentration, after 60-120 minutes of enzymolysis, rapidly heating enzymatic
hydrolysate to 90 DEG C for 20 minutes for
enzyme deactivation treatment; then rapidly
cooling down to
room temperature, carrying out
centrifugation by 3000-4000r / min for 15-20 minutes, taking supernate, adding
trypsin solution into wet macroreticular resin DA201-C according to the proportion of 100g:100-120ml, keeping in a constant temperature table for 180-240 minutes, wherein the temperature is 25 DEG C and the revolving speed is 150-200r / min, eluting sequentially with
ethanol solution of 25%, 50%, 75% and 100% for 120-150 minutes, and collecting eluant for
vacuum drying and concentration. The method is simple and is controllable, an HPLC-
MS analysis result shows that polypeptide molecular weight focus between 1100-1900D, and the
oxidation resistance is stronger than polypeptide obtained through microbial
fermentation.