The invention discloses a safety protection system for a transformer substation. The safety protection system comprises a temperature sensor, an oxygen content sensor, an SF6 sensor, a smoke sensor, acontrol unit, a fan and a safety protection door lock; the safety protection door lock comprises an alarm indicating lamp, an intermediate relay ZJ, a locking electromagnet DSN, a bipolar power switch Q1, a door lock body, a fixed door handle, a key hole, a movable door handle, a movable door handle unlocking auxiliary contact JS and a movable door handle rotating disc; when a locking electromagnet DSN coil is powered off, the locking electromagnet DSN is released and locked to lock the door; and the circle center of the movable door handle rotating disc is fixedly connected with a rotating shaft of the movable door handle, the movable door handle rotating disc rotates along with the movable door handle, a movable door handle unlocking auxiliary contact JS is arranged at a missing part ofthe movable door handle rotating disc, and the movable door handle rotary table rotates along with the movable door handle to trigger the movable door handle unlocking auxiliary contact JS. Accordingto the safety protection system, locking and alarming functions of all room doors are realized, and outdoor workers are effectively prevented from entering rooms by mistake.