The invention relates to bio-cellulose mask essence with a face-lift function. The bio-cellulose mask essence is prepared from the following raw materials in parts by weight: A-phase components: 70-90parts of water, 2-4 parts of mannitol, 0.5-1.5 parts of 1,2-hexanediol and 0.05-0.15 part of ethylene diamine tetraacetic acid; B-phase components: 2-4 parts of a paullinia cupana fruit extract, 1-3parts of a horse chestnut extract, 0.8-1.2 parts of an alchemilla vulgaris extract, 0.7-1.3 parts of a hedera helix extract, 0.5-1.5 parts of an equistetum arvense leaf extract, 0.5-0.7 part of an anthemis nobilis extract and 0.4-0.8 part of a gynostemma extract; C-phase components: 0.5-1.5 parts of N-hydroxysuccinimide, 0.1-0.5 part of hesperidin methyl chalcone, and 0.5-1.5 parts of 5,7-dihydroxyflavone; D-phase components: palmitoyl tetrapeptide-7 and dipeptide-2; an E-phase component: caffeine; the invention further discloses a preparation method of the bio-cellulose mask essence and a mask prepared by utilizing the essence. According to the bio-cellulose mask essence with the face-lift function, the preparation method thereof and the mask prepared by utilizing the essence, retained water accumulated in skin can be eliminated, and excess fat under the skin can also be gradually decomposed; the face-lift effect can be well achieved.