The invention provides an acceleration measurement method based on an anti-magnetic suspension mechanical system, and the method comprises the steps of 1, obtaining an external acceleration a; 2, constructing an acceleration sensitive module, and suspending a diamagnetic mass body by using a magnetic potential well to obtain the position movement delta x of the diamagnetic mass body caused by the external acceleration a; 3, constructing a position measurement module, and realizing the conversion from laser light intensity change delta I to the voltage delta V; and 4, calculating the actual external acceleration a according to the variation of the output measured voltage delta V. According to the invention, an anti-magnetic suspension acceleration sensitive mode is provided, the anti-magnetic suspension mechanical system is different from the existing suspension system, optical suspension, electrostatic suspension, superconducting suspension and the like in the physical principle, does not need the external energy input compared with the electrostatic suspension and the optical suspension, has the lowest parameter noise, reduces the main noise source limiting the acceleration sensitivity, and meanwhile, compared with the superconducting suspension, the anti-magnetic suspension mechanical system can work in a room-temperature or low-temperature environment.