The invention discloses a multi-sound-source positioning method based on Gammatone filter and a histogram. A microphone array is used for collecting a sound source signal, and a sub-band signal is obtained through a Gammatone filter bank, and framing and windowing processing are carried out for converting into a frequency domain; next, a controllable response power value is calculated, a histogramis drawn, the number of the main peak orientation and the secondary peak orientation are counted, and the orientation of the primary and secondary sound sources are estimated. According to the methoddisclosed by the invention, the frequency domains are overlapped with each other and are not separated, so that the phase winding is avoided; the side lobes are inhibited by the average effect of thespatial spectrums of the multiple frequency components, so that the main lobe is protruded, the space between the array elements is not strictly limited to a half wavelength, multi-frame informationis not needed, and the fact that the sound source does not need to be kept static in the continuous multi-frame is not needed to be presumed, and the real-time multi-sound source positioning is realized; and all the sub-band information in the same frame is fused with the histogram to serve as the judgment amount of the azimuth estimation, so that the method is simple and easy to operate, the calculation amount is low, the positioning success rate of the main sound source and the secondary sound sources is remarkably improved, and particularly the positioning success rate of the secondary sound sources is more obvious.