The invention discloses a device and method for detecting color difference of a liquid crystal display screen. The device for detecting color difference of the liquid crystal display screen includes amain casing, a sealed door, a connection plate, a fixing bolt, a first electric push rod, a support plate, a support leg, a guide cylinder, a guide block, a guide rod, a fixing plate, a second electric push rod, a colorimeter body, a fixing column, a fixing rod, a rotating ring, a rotating rod, a fixing shell, an adjusting bolt, a moving block, a fixing frame, a spring, a push rod, a clamping plate, and a placing plate. According to the device and method for detecting the color difference ofthe liquid crystal display screen, the structure is simple, the design is novel, the placement positionof the main casing can be conveniently adjusted according to the needs of a worker, at the same time, stable movement during the adjustment of the main casing is ensured, height of the worker is conveniently met, using is convenient, the liquid crystal display screen placed on the surface of the placing plate is conveniently stabilized and clamped under the action of an arranged clamping mechanism, stability of the placement of the display screen is improved, stable fixation of the liquid crystal display screen during the detection is facilitated for workers, and the detection result is improved.