The invention relates to a preparation method of pleurotus abalonus culture nourishment, which comprises the following components by weight percent: 32-45 of cotton seed hulls, 25-40 of bran, 12-19 of wood chips, 3.6-6.7 of corn flour, 3.5-4 of corn cobs, 0.5-1 of bean cake meal, 0.5-1 of beanstalk meal, 1.7-2 of colza cakes, 0.1-0.3 of oxygenation powder, 0.1-0.3 of calcium carbonate, 0.1-0.3 of bitter salt and 0.5-0.8 of monopotassium phosphate. The cotton seed hulls, the bran, the wood chips, the corn flour, rice bran meal, the corn cobs, the bean cake meal, the beanstalk meal and the colza cakes are combined together in proportion and are poured into a pulverizeer to be pulverized to be granules of about 160 meshes, after the granules are taken out, the oxygenation powder, the calcium carbonate, the bitter salt and the monopotassium phosphate are added in proportion, then the mixture is poured into the pulverizer to be pulverized for 60 min and then is taken out, water is added to the mixture in the proportion of 1:1.8, and then certified culture nourishment is obtained after all materials are piled in a fermentation tank, tightly covered by plastic cloth, compacted and fermented for 72 hours.