The invention relates to the technical field of aquaculture, in particular to a soft-shelled turtle egg artificial incubation method. The method comprises the steps of selecting fertilized soft-shelled turtle eggs, mixing water, coarse sand and wheat straws according to the weight ratio of 1 to 5 to 2 and laying in an incubation pool, moving the soft-shelled turtle eggs to the incubation pool, keeping the incubation temperature at 27 to 28 DEG C, irradiating the soft-shelled turtle eggs with He-Ne lasers in an incubation process, moving larva soft-shelled turtles to a cultivation pool after shell breaking of the soft-shelled turtle eggs, irradiating with fluorescent lamps of the illumination intensity of 1500 to 1700lx for 24 hours every day in a cultivation process, mixing white fish meal, pelvetia silquosa, herba houttyniae, laver powder, powdered Minoru thorns, egg yolk powder, sesame oil, peanut meal and glutinous rice flour according to the weight ratio of 2 to 3 to 7 to 2 to 4 to 2 to 1 to 10 to 3 and adding water and rolling to be granular with the grain size smaller than 1mm in the cultivation process, applying in the cultivation pool, applying twice every day, and applying for 20 to 22g/square meter each time.