Fibroblast Activator, Collagen Production Promoter, Collagen Contraction Promoter, Hyaluronic Acid Production Promoter, ATP Production Promoter, Melanin Formation Inhibitor, and Agent for External Application to the Skin

a technology of collagen production and activation, which is applied in the direction of biocide, drug composition, peptide/protein ingredients, etc., can solve the problems of reducing the elasticity and flexibility of the skin and the like, sagging, and not essentially improving the aging skin,

Inactive Publication Date: 2009-10-08
KANEKA CORP
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  • Summary
  • Abstract
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  • Claims
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Benefits of technology

[0031]Examples of the surfactant include anion surfactants such as soap base, fatty acid soap, higher alkylsulfate, alkyl ether sulfate, N-acylsarcosinate, higher fatty acid amide sulfonate, phosphate, sulfosuccinate, alkylbenzene sulfonate, N-acylglutamate, higher fatty acid ester sulfate, sulfonated oil, POE (polyoxyethylene) alkyl ether carboxylate, POE alkylallyl ether carboxylate, α-olefin sulfonate, higher fatty acid ester sulfonate, secondary alcohol sulfate, higher fatty acid alkylol amide sulfate, lauroyl monoethanol amide succinate, N-palmitoyl aspartate ditriethanolamine, casein sodium and the like, cation surfactants such as alkyltrimethylammonium salt, dialkyldimethylammonium salt, alkylpyridium salt, alkylquaternaryammonium salt, alkyldimethylbenzylammonium salt, alkylisoquinolinium salt, dialkylmorpholium salt, POE alkylamine, alkylamine, polyamine fatty acid derivative, amylalcohol fatty acid derivative, benzalkonium chloride, benzethonium chloride and the like, ampholytic surfactant such as imidazoline surfactant, betaine surfactant and the like, lipophilic nonionic surfactants such as sorbitan fatty acid ester, glycerin fatty acid ester, propylene glycol fatty acid ester, hydrogenated castor oil derivative, glycerin alkyl ether, polyoxyethylene• methylpolysiloxane copolymer and the like, lipophilic nonionic surfactants such as POE sorbitan fatty acid ester, POE sorbit fatty acid ester, POE glycerin fatty acid ester, POE fatty acid ester, POE alkyl ether, POE alkyl phenyl ether, POE-POP alkyl ether, tetraPOE•tetraPOP ethylenediamine condensate, POE hydrogenated castor oil derivative, POE beeswax-lanolin derivative, alkanolamide, POE propyleneglycol fatty acid ester, POE alkylamine, POE fatty acid amide, sucrose fatty acid ester and the like, and the like.
[0032]Examples of the oils include animal vegetable oil and hardened oil thereof such as avocado oil, olive oil, sesame oil, camellia oil, evening primrose oil, turtle oil, macadamia nut oil, corn oil, mink oil, rapeseed oil, egg-yolk oil, apricot kernel oil, wheat germ oil, sasanqua oil, castor oil, linseed oil, safflower oil, cottonseed oil, perilla oil, soybean oil, peanut oil, carmelia sinesis oil, Japanese torreya seed oil, rice bran oil, tung oil, jojoba oil, cacao butter, palm oil, horse oil, palm oil, elaesis guineensis kernel oil, beef tallow, mutton tallow, lard, lanolin, whale wax, beeswax, carnauba wax, Japan wax, candelilla wax, squalane and the like, mineral oils such as liquid paraffin, vaseline and the like, synthetic triglycerols such as tripalmitate glycerin and the like, other oily components and the like.
[0033]Examples of ...

Problems solved by technology

Furthermore, since collagen decreases as one grows old, it is considered one cause of angioembrittlement, decreased inelasticity and flexibility of the skin and the like.
Particularly, with regard to the skin, it is known that the action of fibroblast becomes weak as one grows old, which weakens the power of the cells to pull collagen fiber (collagen contraction force), resulting in inelasticity of the skin and sagging.
While many cosmetics containing, as an aged skin-improving agent, collagen or hyaluronic acid have been proposed, they merely improve surface-moisturizing effect, and do not essentially improve the aging skin.
As the situation stands, however, the treatment of aging skin has not been accomplished yet.
On the other hand, such aging symptoms of human skin, particularly wrinkles and sagging, are also considered to mainly occur due to the degraded function of skin fibroblast an...

Method used

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  • Fibroblast Activator, Collagen Production Promoter, Collagen Contraction Promoter, Hyaluronic Acid Production Promoter, ATP Production Promoter, Melanin Formation Inhibitor, and Agent for External Application to the Skin
  • Fibroblast Activator, Collagen Production Promoter, Collagen Contraction Promoter, Hyaluronic Acid Production Promoter, ATP Production Promoter, Melanin Formation Inhibitor, and Agent for External Application to the Skin
  • Fibroblast Activator, Collagen Production Promoter, Collagen Contraction Promoter, Hyaluronic Acid Production Promoter, ATP Production Promoter, Melanin Formation Inhibitor, and Agent for External Application to the Skin

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0047]The present inventors performed a fibroblast activating action evaluation test by the MTT reduction method and using normal skin fibroblasts derived from human (see TIM Mosmann; Journal of Immunological Methods p 55-63, 1983).

[0048]Using DMEM (Gibco) containing 5% FBS (fetal bovine serum; purchased from NICHIREI CORPORATION), normal skin fibroblasts derived from human (manufactured by KURABO INDUSTRIES LTD.) were plated on a 96 well plate at a density of 2×104 cells / well, and cultured at 37° C., 5% CO2 for 24 hr. After removing the medium, the cells were washed with PBS(−) (NISSUI PHARMACEUTICAL CO., LTD.), the medium was changed to DMEM containing 1% FBS supplemented with each concentration of conagenin, and the cells were cultured at 37° C., 5% CO2. The blank was MEM containing 1% FBS, which was free of test specimen. After culture for 48 hr, the absorbance at 550 nm was measured by MTT reduction method, based on which the MTT reduction amount was determined. The results are...

example 2

Type I Collagen Production Promoting Effect of Conagenin on Human Skin Fibroblasts

[0050]Normal human skin fibroblasts (manufactured by KURABO INDUSTRIES LTD.) were added to a 24 well plate at 5×104 cells per well. Using Medium 106S medium (manufactured by KURABO INDUSTRIES LTD.) containing 2% FBS, the cells were cultured under an atmosphere of 95% (V / V) air-5% (V / V) carbon dioxide gas at 37° C. for 24 hr. The medium was changed to Medium 106S medium (free of FBS) containing each sample, and the cells were cultured under the same conditions for 24 hr. After the completion of culture, the culture supernatant was taken to evaluate the type I collagen biosynthesizability and, to count the cells, the cells were harvested by a trypsin treatment. The samples used were two kinds of conagenin contents of 0.1 μM, 1 μm, 10 μM, 100 μM, 1 mM (final concentration), and magnesium L-ascorbyl phosphate (manufactured by Wako Pure Chemical Industries, Ltd., final concentration 100 μM) as a positive co...

example 3

Collagen Contraction Promoting Effect of Conagenin for Human Skin Fibroblasts

[0052]A collagen solution (collagen was used trade name I-AC manufactured by KOUKEN Co., Ltd.) dissolved in normal human skin fibroblasts (manufactured by KURABO INDUSTRIES LTD., 1×105 cells / ml) was prepared on ice according to the explanation insert attached to the product, and the collagen was gelled in 6 wells at 37° C. Thereafter, 0.25% FBS / DMEM medium containing a sample (PBS as conagenin or negative control) was added. The gel was detached from the dish wall, and the level of contraction of the gelled collagen was examined. As a positive control, DMEM medium containing 10% FBS instead of 0.25% FBS was used. The medium was changed every 2 days and, one week later, the medium was removed by suction. The diameter of the collagen gel was measured, the area was calculated, and the area ratio was compared to that of the negative control as 1. The results are shown in Table 3.

TABLE 3collagen gel area ratione...

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Abstract

The present invention is a fibroblast activator, collagen production promoter, collagen contraction promoter, hyaluronic acid production promoter, ATP production promoter or melanin formation suppressor, which contains, as an active ingredient, at least one kind of compound selected from the group consisting of conagenin, a conagenin derivative, and a pharmaceutically acceptable salt thereof, as well as a composition (skin external preparation, cosmetic, pharmaceutical product or food, which is a composition for the improvement of wrinkles or a whitening composition) containing such activator, promoter or melanin formation suppressor.
According to the present invention, a safe skin external preparation, cosmetic (cosmetic composition), pharmaceutical product or food, which shows a high wrinkle improving effect, can be provided, and a safe skin external preparation, cosmetic (cosmetic composition), pharmaceutical product or food, which shows a high whitening effect can be provided.

Description

TECHNICAL FIELD[0001]The present invention relates to a fibroblast activator, a collagen production promoter, a collagen contraction promoter, a hyaluronic acid production promoter, an ATP production promoter, a melanin formation suppressor and a skin external preparation, as well as cosmetics, which are safe and highly effective.BACKGROUND ART[0002]With increasing population of aged people in recent years, prevention of skin aging evidenced by wrinkles, pigmented spot and the like that increases with aging, i.e., so-called antiaging, has been actively studied.[0003]The skin is mainly divided into epidermis, dermis and subcutaneous tissue. Particularly, the fibroblast constituting dermis produces proteins such as collagen and the like and glycosaminoglycans such as hyaluronic acid and the like, forms a binding tissue (extracellular matrix), and plays an important role for the homeostasis of the skin.[0004]Collagen is a protein occupying about ⅓ of the protein in a living body, and i...

Claims

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Application Information

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IPC IPC(8): A61K31/195
CPCA61K8/44A61Q19/08A61Q19/02A61K31/195A61P17/16
Inventor TOMONO, JUNNAKAI, TAKAHISAFUJII, TOSHIHIDE
Owner KANEKA CORP
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