The invention provides a carrier
assembly carrying a
gene element combination, a recipient
cell library, a preparation and
screening method and an application, the recipient
cell library is formed byfusing cells and the carrier
assembly, and the carrier
assembly at least carries three
gene elements, respectively a plurality of first
gene elements encoding one or more idiotypic synNotch receptors,respectively; a second gene element carrying one or more gene loops; a third gene element encoding one or more idiotypic chimeric
antigen receptors. Wherein, when the first
genetic element encodes one idiotypic synNotch
receptor, the third
genetic element must
encode at least three idiotypic chimeric
antigen receptors, and when the third
genetic element encodes one idiotypic
chimeric antigen receptor, the first genetic element must
encode three idiotypic synNotch receptors. The gene loop is pre-programmed, a regulatory homeopathic factor is combined with a
transcription factor, upon activation of the synNotch
receptor encoded by the first gene element, the
chimeric antigen receptor encoded by the third gene element is controllably expressed.