The invention relates to the technical field of
nucleic acid detection, and discloses a
nucleic acid integrated detection device based on a flexible structure and a push-pull structure, which comprises a detection main body, an upper cover, a bottom shell and an
extrusion block arranged between the upper cover and the bottom shell, the detection main body is arranged in an accommodating cavity between the upper cover and the bottom shell; the detection main body comprises a sample introduction tube, a
nucleic acid extraction-free
reagent chamber, a first
buffer solution chamber, a nucleic acid amplification
reagent chamber, a second
buffer solution chamber, a uniform
mixing chamber, a sample loading groove and
chromatography test paper which are connected in sequence, wherein the nucleic acid extraction-free
reagent chamber, the first
buffer solution chamber, the nucleic acid amplification reagent chamber, the second buffer solution chamber and the uniform
mixing chamber are flexible reagent chambers; the head end of the sample loading groove is connected with the uniform
mixing chamber, and the
tail end of the sample loading groove is connected with the
chromatography test paper; and the
extrusion block can move from one end of the upper cover to the other end and is used for extruding the liquid in the detection main body and forcing the liquid to flow downstream. The liquid is extruded to flow downstream only when the
extrusion block is manually pushed to move, so that the
controllability of the detection process can be realized.