The invention relates to a tenoning clamping mechanism, and belongs to the technical field of wood industry processing. The tenoning clamping mechanism comprises a worktable frame, a pressing plate, a first air cylinder and a second air cylinder, wherein the worktable frame is used for supporting a tenoning workpiece, the pressing plate is integrally formed, and is used for providing upward pressing force and lateral pressing force for the tenoning workpiece, the first air cylinder and the second air cylinder are used for providing pressing power, the worktable frame is in an L shape, the longitudinal edge of the worktable frame is provided with a movable area, an air cylinder shaft of the first air cylinder transversely penetrates through the movable area and is connected with the pressing plate, the second air cylinder is arranged at the longitudinal edge of the worktable frame, an air cylinder shaft of the second air cylinder longitudinally acts on the shaft of the first air cylinder, and the first air cylinder and the pressing plate are moved along the longitudinal axis direction of the movable area. The tenoning clamping mechanism has the advantages that the structure is simple; by arranging the integral forming pressing plate, the number of pressing plates is reduced, the operation of operators is convenient, the upward pressing force, lateral pressing force and edge and corner force are simultaneously provided, the tenoning workpiece is clamped, and the tenoning accuracy is provided.