The invention, which belongs to the technical field of measurement, particularly relates to a detection method for a trace amount of dust based on microscopic image processing. The method comprises the following steps: step 1, collecting an image of eachdust particle on a certain surface by using a microscopic imaging system; step 2, performing gray processing on the dust particle images; step 3,performing illumination correction ongrayscale images by using a block background extraction method; step 4, carrying out median filtering processing on the images after illumination correction; step5, drawing gray histograms for the grayscale images; step 6, carrying out image edge detection on the images by adopting a Prewitt operator; step 7, performing binary image processing such as expansion and corrosion on the images; step 8, performing feature extraction on the images, and carrying out statistics on the number of pixel points occupied by each dust particle so as to obtain a pixel area of each dust particle; and step 9, deducing the volume of the dust particle according to the pixel area of the dust particle and acquiring the mass of each dust particle based on the volume and density to obtain the total mass of the dust particles.