The invention discloses a sampler for biotechnology separation, which comprises a sampling cylinder and a sample tube. A sampling probe is fixedly installed at the bottom of the sampling cylinder, and an inlet funnel is arranged on the top of the sampling cylinder. A buffer, and a sampling inlet pipe and a sampling outlet pipe are respectively fixedly installed on both sides of the buffer, and a pull rod is arranged inside the sampling cylinder, and the top of the pull rod is provided with an embedding protrusion, and the top of the sample tube is provided with an embedding groove, The outer surface of the embedding protrusion and the inner surface of the embedding groove are engraved with mutually engaging threads, the outer surface of the sampling probe is provided with mounting threads, a sampling room is arranged inside the sampling probe, and a closing plug is installed at the bottom of the sample tube , and a receiving baffle is fixedly installed at the connection between the inside of the sampling probe and the sampling cylinder to realize periodic and continuous sampling at a fixed point and timing, and retain the state of the sample when it is collected without destroying the original properties of the sample and the product state during the separation process .