The invention discloses a one-way valve assembling and detecting machine. The one-way valve assembling and detecting machine comprises a one-way valve to be assembled, an assembling rotating disc, a water outlet end assembling and detecting mechanism, a rubber sleeve assembling and machining mechanism, a water inlet end assembling and detecting mechanism, a secondary press fitting mechanism, a pressure starting and leakage detecting mechanism and a finished product collecting mechanism, wherein the assembling rotating disc, the water outlet end assembling and detecting mechanism, the rubber sleeve assembling and machining mechanism, the water inlet end assembling and detecting mechanism, the secondary press fitting mechanism, the pressure starting and leakage detecting mechanism and the finished product collecting mechanism are sequentially arranged. The one-way valve assembling and detecting machine has the beneficial effects that the assembling process of the one-way valve is divided into the process in which three portions, namely a water outlet end, the rubber sleeve and the water inlet end, of the one-way valve are assembled and detected, so that automatic assembling of the one-way valve is realized, the working efficiency is improved, meanwhile, the high labor cost expenditure is reduced, the pressure starting and leakage detecting mechanism is arranged to be used for performing quality detection on the assembled one-way valve, it is guaranteed that the quality of a product meets the delivery requirement, and the quality of the machining technology is improved.