The invention discloses a Tai Kong distiller's yeast production testing method. The Tai Kong distiller's yeast production testing method comprises the following steps: S1) preparation of raw materials: preparing rhizopus distiller's yeast, aspergillus niger distiller's yeast, aspergillus oryzae distiller's yeast, sorghum, wheat, barley and pea as the experiment raw materials, and S2) preparation of a starter blank: matching the wheat, barley and pea according to a ratio, then respectively adding the rhizopus distiller's yeast, aspergillus niger distiller's yeast, and aspergillus oryzae distiller's yeast, adding water and uniformly stirring the materials, performing artificial stepping to prepare the starter blank, examining the starter blank, and performing a next operation after the starter blank is qualified after examination, and recording the detection data. The Tai Kong distiller's yeast and common Daqu are subjected to contrast testing, influence of the Tai Kong distiller's yeastand common Daqu on wine quality and the wine yield rate can be compared, a proper amount of the Tai Kong distiller's yeast during a wine brewing process is determined, grain consumption is reduced, wine quality and the wine yield rate can be increased, the test data is recorded, the data is integrated, and the test data can be conveniently and timely extracted.