The invention provides a delay detection device and a detection method of the delay detection device. The delay detection device and the detection method are based on automatic transmitting and automatic receiving. The detection method includes the steps that a starting module receives external control to trigger spread spectrum transmitting signals, a set of signals coded in a spread spectrum mode through pseudo-random codes are generated, and a timing mark is generated; signals generated in the mode that the spread spectrum signals are coupled through a radio-frequency channel module are transmitted to an A/D conversion circuit, A/D conversion is carried out on the signals through the A/D conversion circuit, then the signals are transmitted to a digital correlator, and the digital correlator completes signal capturing and generates a correlation peak; time between the starting mark and the correlation peak is calculated in real time through a calculating circuit, and delay of the signals flowing through the modules is calculated. The delay detection device and the detection method can be widely applied to systems, such as a burst communication system, with delay estimation, delay detection has the advantages of being automatic, adaptive and the like, the batch assembling performance and the use performance of products are accordingly improved, and the delay detection device and the detection method are particularly suitable for the batch-assembling products.