39 results about "Viricidal activity" patented technology

The invention discloses a virulent bacteriophage vBValSR36H with a host being vibrio alginolyticus and application of the virulent bacteriophage vBValSR36H, and relates to the technical field of microbial control. The invention provides a bacteriophage capable of efficiently cracking Vibrio alginolyticus ATCC 17749T, the bacteriophage is named as vBValSR36H, the preservation number is 66248-B1, and the preservation address is Institute of Microbiology, Academy of Sciences in Guangdong Province. The vibrio alginolyticus bacteriophage vBValSR36H provided by the invention has good environmental stability and certain bactericidal activity, and has a relatively wide environmental adaptation range and relatively long survival time. The bacteriophage vBValSR36H provided by the invention can specifically and effectively kill, prevent and control related vibrio diseases, meanwhile, virulence factor genes and drug-resistant genes are not detected in the bacteriophage, and the bacteriophage vBValSR36H has the advantages of no toxic or side effect, high safety, environmental friendliness and wide application prospect. Therefore, the vibrio alginolyticus bacteriophage vBValSR36H can be completely applied to killing and / or preventing vibrio alginolyticus for non-disease diagnosis and treatment purposes.

The present invention deals with a heat and moisture exchange filter with biocidal activity for medical ventilation equipment that comprises polymeric cellulose fibers impregnated with silver nanoparticles (AgNPs). The heat and moisture exchanger filter can be used in intubated patients under artificial ventilation in anesthesiology and intensive care and allows robust replacement, a fundamental utility for protection against nosocomial cross-contamination infections caused by microorganisms, such as bacteria, fungi, yeasts and viruses.The invention also deals with the method of preparing the heat and moisture exchange filter with biocidal activity and the use of silver nanoparticles.

The invention discloses a broad-spectrum antibacterial peptide as well as a screening method and application thereof, and belongs to the technical field of antibacterial peptides, a bioinformatics method is mainly adopted for searching a new antibacterial peptide, then amino acid sequence optimization is carried out, and finally the antibacterial peptide with a better antibacterial effect is obtained. Sterilization activity evaluation is carried out by using various known strains with drug resistance, and the result shows that the antibacterial peptide provided by the invention has broad-spectrum antibacterial activity on drug-resistant bacteria, more comprehensive antibacterial effect and wider indications; the compound has the advantages of no hemolysis, low cytotoxicity, efficient in-vivo antibacterial activity and high stability, and has application safety; the compound has a wide application prospect of being developed into a human body antibacterial drug.

The invention belongs to the technical field of biological medicines, and particularly relates to a granzyme B expression system as well as a preparation method and application thereof. According to the invention, granzyme B with bactericidal activity is screened out, a corresponding expression vector is designed by adopting circular RNA, a bacterium-specific ribosome binding site (RBS) is taken as a protein translation regulation and control element, and a Lipid nano-particle (LNP) is taken as a delivery vector, so that efficient delivery of the circular RNA for coding GZMB and specific expression of GZMB in bacteria are realized. According to the strategy, efficient delivery and specific expression of circRNA for coding GZMB in bacteria are achieved, and a safe and accurate scheme is provided for GZMB antibacterial treatment.

This invention relates to recombinant lyases and variants thereof, which have improved stability or bactericidal activity relative to the recombinant lyases. This application also relates to pharmaceutical compositions comprising the recombinant lyases and variants thereof, and their use for the prevention and / or treatment of bacterial infections (e.g., bacterial vaginosis).

The invention discloses a fusion protein containing bacteriophage lyase and LPS (lipopolysaccharide) binding peptide, and further modifications including amino acid mutation, combination mode change and chemical modification are carried out on the fusion protein. The bactericidal activity of the bacteriophage lyase is improved, and meanwhile, the in-vivo stability is improved. The fusion protein provided by the invention is suitable for preventing and treating diseases caused by bacterial infection, especially gram-negative bacterial infection.

The invention discloses a polypeptide and an application of the polypeptide in preparation of antibiotics. The amino acid sequence of the polypeptide is LRDLVCYCRS10 RGCKGRERMN20 GTCRK25. According to the invention, it is found that L-Crp1 tends to form a double alpha-spiral structure in a membrane environment, the double alpha-spiral structure can fully expose alkaline residues, and the double alpha-spiral structure closely interacts with a microbial membrane and mediates membrane permeation of polypeptide, which is the key of the strong bactericidal activity of L-Crp1 to escherichia coli; however, the disordered segment of the C end of the L-Crp1 peptide chain does not participate in the interaction with the membrane, the polypeptide obtained after the segment is cut off still has a complete double-alpha-spiral structure, the bactericidal activity of the polypeptide is equivalent to that of the L-Crp1, but the length of the peptide chain of the polypeptide is shorter than that of the L-Crp1, so that the production cost is greatly reduced (reduced by 30-40%), and the polypeptide has greater application potential than the L-Crp1.

Metal nanoparticle agglomerates may convey biocidal activity to surfaces upon which they are deposited and become adhered, such as various air filtration media. Air filtration media may comprise a plurality of fibers having a plurality of metal nanoparticle agglomerates adhered thereto. The metal nanoparticle agglomerates may comprise a plurality of fused, partially fused, or unfused metal nanoparticles that are associated with one another upon a surface of the plurality of the fibers. Suitable metal nanoparticles for promoting biocidal activity against various pathogens, such as viruses and bacteria, may include copper nanoparticles and / or silver nanoparticles. Masks, inline filters, and air filtration systems may incorporate the air filtration media.

The present application relates to a kind of pseudomonas aeruginosa engineering bacteria DUEC construction method and application.The DUEC engineering strain construction method of the present application is: response to kinase coding gene retS and sheath protein coding gene tssB2, tssB3 Knockout, H1-T6SS secretory effector protein Tse1-Tse4, Tse6-Tse8 catalytic site mutation, Tse5 Coding gene knockout.DUEC engineering strain can not only constitutively express and assemble H1-T6SS, but also can respond to adjacent sister cell source T6SS attack, assemble H1-T6SS at the position of being attacked.The present application constructs a series of effector protein combination inactivation mutant by gene knockout or catalytic site mutation method.Microscope observation and statistical analysis results show that no single effector protein is necessary for T6SS attack and assembly.By bacterial competition experiment, it is verified that all effector protein inactivation mutant 8eff c Lose T6SS + Vibrio cholerae and T6SS + Aeromonas dhaka's bactericidal activity, but 8eff c Can respond to T6SS physical attack, Tse6 N -Cre is specifically delivered to T6SS + In Vibrio cholerae.

The present application relates to the field of agricultural technology, and particularly relates to an amine group-containing alstoniascholaris alkaloid derivative, a preparation method and application thereof. The amine group-containing alstoniascholaris alkaloid derivative comprises a compound as shown in formula (I): wherein R comprises at least one of C3-C12 linear alkyl, C3-C12 branched alkyl, C3-C6 cycloalkyl, C3-C6 cycloalkylmethyl, C2-C6 aminoalkyl, tetrahydropyrrole group, piperidine group, piperidinylmethyl, N-methylpiperidine group and N-methylpiperidinylmethyl. The amine group-containing alstoniascholaris alkaloid derivative has good plant virus control and good fungicidal activity.

The present invention provides a kind of bacterial microenvironment responsive immunomodulatory antimicrobial peptide and its preparation method and application, belong to the field of bioengineering, its sequence is shown in SEQ ID No.1, its N-terminus is connected to the carboxyl group of indole 3-propionic acid, and the phenylalanine in the sequence is D-phenylalanine. Also disclosed is the application of the antimicrobial peptide of the present invention in the preparation of a drug for treating infectious diseases caused by Escherichia coli. The antimicrobial peptide of the present invention does not have direct bactericidal activity under physiological conditions, has a good antibacterial effect (16 μM) on Escherichia coli under acidic conditions, and has a macrophage immune response, promotes the phagocytic ability of macrophages to bacterial clusters. Therefore, the antimicrobial peptide of the present invention has selective bactericidal function, can coordinate the innate immune system, can effectively reduce bacterial selective pressure, and solves the problem of drug resistance.

The application discloses a Pseudomonas aeruginosa single-stranded RNA bacteriophage and application thereof, and belongs to the technical field of bioengineering, wherein the Pseudomonas aeruginosa single-stranded RNA bacteriophage is named Pseudomonas aeruginosa phage Ppa61, is preserved in the China Center for Type Culture Collection, and has a preservation number of CCTCC No: M 20231334. The bacteriophage has strong bactericidal activity and a wide host spectrum on Pseudomonas aeruginosa, is specific to Pseudomonas aeruginosa, cannot kill other bacteria, and can be used alone or in combination with other substances, thereby providing a safe and non-toxic bacteriophage killing product for in-vivo and in-vitro Pseudomonas aeruginosa infection treatment, Pseudomonas aeruginosa disinfection and purification in aquaculture and other environments.

The invention provides an SP-1 polypeptide and application of a coding gene of the SP-1 polypeptide in inhibition of tight reaction and antibiosis, and belongs to the technical field of polypeptides. The amino acid sequence of the spider-derived SP-1 polypeptide is as shown in SEQ ID NO: 1. According to the present invention, the SP-1 polypeptide can specifically inhibit Escherichia coli and pseudomonas aeruginosa tight reaction key small molecule (p) ppGpp synthase activity, strong antibacterial activity is represented, and the MIC of the SP-1 polypeptide on Escherichia coli and pseudomonas aeruginosa is 2.4 [mu] M and 1.5 [mu] M respectively, and is significantly lower than the MIC (14.5 [mu] M and 12.3 [mu] M) of kanamycin of a positive control group under the same experiment condition; in addition, the SP-I peptide shows efficient bactericidal activity, the minimum bactericidal concentration of the SP-I peptide to escherichia coli and pseudomonas aeruginosa is 2.0 mu M, and the SP-I peptide can effectively inhibit generation of escherichia coli and pseudomonas aeruginosa biological membranes. Therefore, the SP-1 polypeptide can be used as an active ingredient to be applied to preparation of antibacterial drugs for inhibiting multidrug resistance bacteria.

The present invention relates to the field of agricultural protection technology, in particular to a leucine salt derivative and its application in preventing and controlling plant viruses and sterilizing. The leucine salt derivative includes a compound represented by formula (I): #imgabs0# wherein R 1 Includes at least one of hydrogen, methyl, methoxy, F, Cl, Br, I and cyano; R 2 Includes at least one of hydrogen, methyl, methoxy, F, Cl, Br, I, trifluoromethyl and methoxycarbonyl; R 3 including at least one of methyl, cyclopropylmethyl, cyclohexylmethyl, 2,2-dimethylpropyl and benzyl; R 4 The leucophylline salt derivatives have good plant virus prevention and control and good fungicidal activity.

The application discloses application of mulberroside D in synergistic polymyxin antibacterial activity, and belongs to the technical field of medicines, and particularly relates to application of mulberroside D in synergistic polymyxin antibacterial activity. The application discloses application of mulberroside D in preparation of a product for enhancing antibacterial activity and / or bactericidal activity of an antibacterial drug. The mulberroside D can effectively synergize in-vivo and in-vitro antibacterial activity of polymyxin on multi-drug resistant bacteria, can produce synergistic antibacterial effect, and provides a new treatment strategy for clinical treatment of bacterial infectious diseases.

The invention discloses a group B meningococcus vaccine composition as well as a preparation method and application thereof. The vaccine composition comprises a factor H binding protein (fHbp) fusion protein and an outer membrane vesicle (OMV) wherein the factor H binding protein fusion protein comprises a meningococcal heparin binding protein antigen (NHBA) or a variant thereof, and an operably linked fHbp or a variant thereof. The vaccine composition can induce an organism to generate a functional antibody with bactericidal activity, has bactericidal activity on three variant strains, can induce the organism to generate higher protective response, and improves the bactericidal coverage rate of the vaccine on domestic B group strains.

The invention relates to a group of polypeptides with rapid bactericidal activity and application thereof, and the polypeptides are amphiphilic polypeptides and have a cation alpha-helical structure. The polypeptide specifically comprises a C22-01 polypeptide, a C22-02 polypeptide, a C22-03 polypeptide, a C22-04 polypeptide and a C22-05 polypeptide, wherein the sequence of the C22-01 polypeptide is shown as SEQ ID NO.1, the sequence of the C22-02 polypeptide is shown as SEQ ID NO.2, the sequence of the C22-04 polypeptide is shown as SEQ ID NO.3, the sequence of the C22-05 polypeptide is shown as SEQ ID NO.4, and the sequence of the C22-05 polypeptide is shown as SEQ ID NO.5. The carboxyl terminals of the polypeptides are subjected to amidation (-NH2) modification.

This invention relates to a novel natural microbial metabolite: Fcl-8, derived from the fermentation broth of *Xenorhabdus budapestensis* XBD8. Purified Fcl-8 is a snow-white, flocculent crystal, highly soluble in water, as shown in Figure 1. The chemical structural formula of Fcl-8 is shown in Figure 2, with the chemical formula C1. 57 H 102 N 14 O 12 With a molecular weight of 1175.78744, its typical secondary mass spectrometry information is shown in Figure 3. Fcl-8 is derived from a natural microbial metabolite, has a novel structure and high biological activity, and possesses great potential for development into a novel biopesticide.

The invention relates to fast-acting viricidal compositions and methods of using the same, wherein the compositions are capable of removing soil and effectively inactivating a wide variety of viral pathogens, particularly small, non-enveloped viruses such as poliovirus and norovirus. The compositions are especially useful on hard surfaces, and can easily be applied to a service to provide rapid viricidal efficacy.

The invention provides a lyase general formula, and aiming at the general formula, the activity and the function of the lyase can be further screened. 24 lyases are preliminarily screened out, more specifically, the characteristics of 9 lyases are explored, and the 9 lyases have efficient bactericidal activity and can split various staphylococcus, streptococcus and / or enterococcus; the compound has good environmental resistance and stability, is stable in activity in solutions with different temperatures, different NaCl and different pH values, and is not easy to inactivate in immune serum; and local infection and systemic infection can be effectively treated. The lyase and variants thereof are expected to become antibacterial agents for removing bacteria, preventing bacterial infection or treating bacterial infection (staphylococcus, streptococcus and / or enterococcus bacteria).

A use and a method of conferring permanent disinfectant properties on a surface of any material by distribution on it of a disinfectant solution made of a mixture of certain lipids with biocidal properties with suitable volatile solvent; wherein after distribution on the surface, the solvent evaporates progressively, leaving a very thin layer of homogeneously arranged lipids on it. This lipid layer renders the surface active in the killing and deactivation of viruses and bacteria. Furthermore, a method for disinfecting a material can be achieved by applying the disinfectant composition to a treated substrate having biocidal activity obtainable with the method.

The invention discloses a vibrio cantoniensis phage VCP13AVCGD9 as well as a composition and application of the vibrio cantoniensis phage VCP13AVCGD9, the vibrio cantoniensis phage VCP13AVCGD9 is preserved in Guangdong Microbiological Culture Collection Center on November 24, 2025, the preservation number of the vibrio cantoniensis phage VCP13AVCGD9 is GDMCC NO. 67344-B1, and the vibrio cantoniensis phage VCP13AVCGD9 is classified and named as Vibrio campbelli phage VCP13AVCGD9. The vibrio canetinii bacteriophage VCP13AVCGD9 provided by the invention has good acid-base stability, can still keep relatively high titer after being treated for 96 hours under the condition that the pH value is 4.0-8.0, is beneficial to continuously keeping bactericidal activity in a culture water body, and acts on vibrio canetinii in animal gastrointestinal tracts in a targeting manner.

The invention discloses an engineered PVC protein compound for targeted removal of intracellular staphylococcus aureus as well as a preparation method and application of the engineered PVC protein compound, and belongs to the fields of genetic engineering, microbiology and drug delivery. Genetic engineering modification is carried out on PVC, a natural recognition domain of tail fiber protein of the PVC is replaced by a staphylococcus aureus receptor binding structural domain (SRapid BR), and meanwhile, natural toxin protein loaded in an inner cavity of the PVC is replaced by human beta-defensin-3 (HBD-3) with efficient bactericidal activity. The engineered compound can directly deliver HBD-3 into infected cells, effectively remove intracellular staphylococcus aureus which is difficult to kill by conventional antibiotics, and relieve inflammatory response caused by infection. The invention provides a brand-new treatment strategy for resisting intracellular bacterial infection with high targeting, and shows a wide application prospect as a programmable protein delivery platform.

The invention provides a bacterial microenvironment response type immunoregulation antibacterial peptide as well as a preparation method and application thereof, and belongs to the field of bioengineering, the sequence of the bacterial microenvironment response type immunoregulation antibacterial peptide is as shown in SEQ ID No.1, the N end of the bacterial microenvironment response type immunoregulation antibacterial peptide is connected with carboxyl of indole-3-propionic acid, and phenylalanine in the sequence is D type phenylalanine. Meanwhile, the invention further discloses application of the antibacterial peptide in preparation of drugs for treating infectious diseases caused by escherichia coli. The antibacterial peptide provided by the invention does not have direct bactericidal activity under physiological conditions, has a good antibacterial effect (16 [mu] M) on escherichia coli under acidic conditions, and also has the effects of regulating the immune response of macrophages and promoting the phagocytic ability of the macrophages on bacterial clusters. Therefore, the antibacterial peptide provided by the invention has a selective sterilization function, can coordinate the innate immune system, can effectively reduce the selective pressure of bacteria, and solves the problem of drug resistance.

The present application relates to the technical field of antibacterial drugs, in particular to a small-molecule antibacterial polypeptide, a preparation method and uses thereof. The antibacterial peptide provided by the present application has stronger bacteriostatic activity than most other derivative polypeptides and antibacterial polypeptides disclosed in existing reports. The bactericidal activity evaluation was carried out using a plurality of strains known to have drug resistance, and the results showed that the antibacterial peptide provided by the present application has broad-spectrum bacteriostatic activity on drug-resistant strains, the bacteriostatic effect is more comprehensive, and the indications are more extensive.

The present application relates to the technical field of antibacterial drugs, in particular to a small-molecule antibacterial polypeptide, a preparation method and uses thereof. The antibacterial peptide provided by the present application has stronger bacteriostatic activity than most other derivative polypeptides and antibacterial polypeptides disclosed in existing reports. The bactericidal activity evaluation was carried out using a plurality of strains known to have drug resistance, and the results showed that the antibacterial peptide provided by the present application has broad-spectrum bacteriostatic activity on drug-resistant strains, the bacteriostatic effect is more comprehensive, and the indications are more extensive.

The invention discloses a salmonella paratyphi targeting antibacterial peptide as well as a preparation method and application thereof, and belongs to the field of bioengineering, and the sequence of the antibacterial peptide is as shown in SEQ ID No.1; the invention further discloses application of the antibacterial peptide targeting the salmonella paratyphi to preparation of a medicine for treating salmonella paratyphi infectious diseases. The antibacterial peptide disclosed by the invention has efficient targeted bactericidal activity on salmonella paratyphi, and has no toxicity on human red blood cells, so that the antibacterial peptide has the potential of becoming a feed antibiotic substitute.

The invention relates to fast-acting viricidal compositions and methods of using the same, wherein the compositions are capable of removing soil and effectively inactivating a wide variety of viral pathogens, particularly small, non-enveloped viruses such as poliovirus and norovirus. The compositions are especially useful on hard surfaces, and can easily be applied to a service to provide rapid viricidal efficacy.

The present invention relates to an asymmetric aromatic diselenide compound and the use thereof in the preparation of pesticides, particularly to the use thereof in the preparation of anti-plant virus agents and fungicides. The asymmetric aromatic diselenide compound of the present invention exhibits a good efficiency on tobacco mosaic virus (TMV) at a concentration of 500 mg / L, achieving an in-vivo inactivation efficiency of 71.6%, an in-vivo protective efficiency of 58.3%, and an in-vivo therapeutic efficiency of 60.1%. The asymmetric aromatic diselenide compound of the present invention exhibits good control effects on tomato early blight, wheat Fusarium head blight, rice blast, pepper Phytophthora blight, rapeseed Sclerotinia rot, cucumber gray mold, rice sheath blight, cucumber wilt, peanut brown spot, apple ring rot, wheat sharp eyespot, southern corn leaf blight, watermelon anthracnose, and rice bakanae disease at a concentration of 50 mg / L, and has a fungicidal activity of up to 92.3%.

The invention discloses a vibrio parahaemolyticus recombinant outer membrane protein OmpU and application thereof.The recombinant protein is the vibrio parahaemolyticus outer membrane protein OmpU, the amino acid sequence of the recombinant protein is shown as SEQ ID NO: 1 or is a variant which has at least 90% identity with SEQ ID NO: 1 and retains immunoprotectiveness, and the protein has high surface exposure, high expression abundance and strong immunogenicity; the generation of a high-titer antibody can be induced and complement-dependent bactericidal activity can be mediated. Experiments show that the protein is highly conservative in vibrio parahaemolyticus and other vibrios, and can be used as a broad-spectrum subunit vaccine, a diagnostic reagent or a candidate antigen of a therapeutic antibody.