The invention relates to an automatic closing floor drain in a clean air-conditioning room, comprising: a drain pipe, a U-shaped water trap, a water outlet pipe, a valve core, a hinge, a side rod, a main rod, a floating ball, a floor drain support grid, and a floor drain tank. The floor drain tank is covered with a floor drain support grid, and the lower part of the floor drain tank is connected to a water outlet pipe, and the water outlet pipe, U-shaped water trap and drain pipe are connected in sequence. One end of the main rod is connected to the hinge on the upper part of the inner wall of the floor drain tank, and the other end is fixedly connected to the floating ball. The middle part of the main rod is fixedly connected to one end of the side rod, and the other end of the side rod is fixedly connected to the spool. The support grid of the floor drain is made of flat steel vertically arranged in a grid shape, and the material of the flat steel is stainless steel. The floor drain water tank is made of 4mm to 8mm steel plate, and the surface is treated with antirust. The invention can automatically close the water outlet pipe when the clean air-conditioned room is not drained, isolate the inside and outside of the clean room, and prevent outdoor air or reptiles from climbing into the clean air-conditioned room from the outside, affecting the air quality of the clean air-conditioned room and further affecting product quality. The structure is simple, and no other materials or energy are consumed during use.