The invention discloses a thermal response type bornyl steam instant micro-needle eye mask and a preparation method and application thereof. The eye mask comprises a micro-needle layer, a middle slow release layer and a heating layer which are connected in sequence, wherein the micro-needle layer contains natural d-borneol. The micro-needle layer takes the natural d-borneol as a penetration enhancer, so that functional components are rapidly absorbed by skin cells, and the using effect of a micro-needle is remarkably enhanced; the heating layer is contained, so that the micro-needle can be heated, the bioavailability of the functional components is improved, the puncture feeling and pain feeling of the micro-needle can be remarkably reduced, the using comfort of the micro-needle is improved, the internal microcirculation of skin can be accelerated, the absorption of the functional components is promoted, and the dissolution rate of the micro-needle is increased; and the middle slow release layer is rich in water-in-oil micro-emulsion, essential oil and moisture in the micro-emulsion can generate steam in the heating process, and the steam can enter the skin through the micro-needle, so that on one hand, the skin moisture is supplemented, and the dissolution of the micro-needle is accelerated, and on the other hand, the essential oil can achieve the effects of awakening the skin vitality, enhancing the skin tolerance and keeping the skin in an optimal state.