The invention discloses a positioning frame for breast examination, which comprises a bed body, a mounting groove, a driving mechanism, a linkage mechanism, a placing groove, a rotating mechanism, a soft cushion, a control mechanism and a lithium battery. The positioning frame can adjust the state that two arms of a patient are lifted to be within the range required by medical staff; then the lifting of the two arms of the patient is controlled through the active driving force of the device, so that the feeling of soreness and swelling when the two arms of the patient are lifted is eliminated, and therefore, by using the device, the pain of the patient during examination is reduced, and the progress of examining the patient by medical staff is also improved.