Patents
Literature
1 results about "Dominant-Negative Mutant" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
A dominant-negative mutation usually means that the resulting protein is has lost a certain part of its function (negative), but it can out-compete the endogenous protein in some way (dominant).
Method for culativating huanglongbing-resistant plant and application thereof
A method and application for cultivating a Huanglongbing-resistant plant is provided. CsPUB21 attenuates a defense response of citrus based on terpene compounds through direct ubiquitination and degradation of a CsMYC2 protein. Resistance of citrus to a pathogen CLas and a spread vector thereof, i.e. Asian Diaphorina citri Kuwayama, can be improved by silencing of a CsPUB21 gene. Infestation of the pathogen CLas can be reduced by overexpression of CsPUB21DN, a dominant negative mutant of PUB21. Additionally, the CLas concentration in leaves of a susceptible plant can be effectively reduced by artificially replacing an amino acid Cys (TGC) at position 39 of a key enzyme activation site of CsPUB21 in the susceptible plant with Ala (GCC) via gene editing. This method and application provide a means to effectively resist the replication and spread of Huanglongbing pathogens, which cause citrus greening.
Owner:INST OF MICROBIOLOGY CHINESE ACAD OF SCI