9 results about "Endarteritis" patented technology

The invention discloses a model building method based on animal endometrial epithelium organoid and application of the model building method, and relates to the technical field of cell engineering and organoid. According to the method, healthy cow endometrial tissue serves as a raw material, a model is built through the steps of pretreatment, enzymolysis, cell cleaning and screening, seed gum culture, passage, cryopreservation resuscitation and the like, and the model is built through Hamp; e, verifying morphological integrity through dyeing, and detecting E-cadherin expression through IHC dyeing to confirm cell specificity. The animal organ model restores the in-vivo three-dimensional microenvironment and species specificity, overcomes the limitation of traditional two-dimensional culture and mouse models, can accurately simulate the pathological process of endometritis, is applied to research on the pathogenesis of endometritis of dairy cows, can also efficiently screen anti-inflammatory drugs and evaluate the drug effect, and has a wide application prospect. A reliable in-vitro tool is provided for disease prevention and treatment, and healthy development of the dairy cow breeding industry is assisted.

This invention belongs to the field of biomedical technology and provides the application of carvacrol combined with astragaloside A in the preparation of drugs for treating endometritis. This invention confirms that carvacrol combined with astragaloside A is effective against *Ralstonia pinnatifida* (…). R. pickettii The combination of carvacrol and astragaloside A in this invention has significant therapeutic benefits in treating mouse endometritis: It exhibits a synergistic antibacterial effect, rapidly reducing body temperature and weight loss in mice, significantly decreasing the uterine index, alleviating tissue congestion and edema, and significantly downregulating pro-inflammatory factors such as TNF-α, IL-1β, and IL-6 while upregulating the anti-inflammatory factor IL-10, thus inhibiting excessive activation of inflammatory signals. Simultaneously, it reduces MDA, increases SOD and GSH levels, and alleviates oxidative stress damage. Therefore, the combination of carvacrol and astragaloside A in this invention can effectively repair the pathological structure of the uterus, with superior effects compared to single drugs, and is safe and has no significant toxicity, providing a safe and drug-free natural alternative for endometritis in livestock.

This invention provides the application of a uterine-derived Sphingomonas DS-28 strain and its metabolites in the treatment of endometritis, relating to the field of microbial technology. The Sphingomonas DS-28 strain provided by this invention possesses uterine colonization ability, good niche adaptability, and significant efficacy in treating Escherichia coli-induced endometritis. Animal experiments show that the preparation of this strain can effectively alleviate uterine redness and swelling, reduce pathological damage, and significantly reduce [the condition / progression]. TNF-alpha , IL-6 , IL-8 It inhibits gene expression and suppresses the NF-κB signaling pathway, exhibiting superior anti-inflammatory and tissue repair effects compared to other Sphingomonas strains C3-25.1, C3-41.1, and C3-42.1. Furthermore, the metabiotic preparation of this strain can also alleviate Escherichia coli-induced endometritis in mice, improve uterine redness and swelling, and reduce pathological damage.

The invention provides a traditional Chinese medicine compound suppository for treating endometritis of livestock and a preparation method of the traditional Chinese medicine compound suppository, and relates to the technical field of veterinary drugs. The traditional Chinese medicine compound suppository consists of povidone iodine, a traditional Chinese medicine composition and a slow-release high polymer material matrix carrier, wherein the traditional Chinese medicine composition is prepared from thirteen traditional Chinese medicine components including philippine flemingia root, common andrographis herb, zanthoxylum dissitum, Chinese angelica, suberect spatholobus stem, motherwort herb, baical skullcap root, honeysuckle flower, weeping forsythia capsule, white paeony root, nutgrass galingale rhizome, heartleaf houttuynia herb and swordlike atractylodes rhizome. Besides the matrix carrier, the traditional Chinese medicine compound suppository only contains povidone iodine and traditional Chinese medicine composition components, the content of the povidone iodine component is low, and side effects are reduced. The traditional Chinese medicine compound suppository is non-toxic, small in irritation to vaginal mucosa of livestock, high in effective rate of treating endometritis of the livestock and good in anti-inflammatory effect. In the preparation process of the traditional Chinese medicine compound suppository, no organic solvent is used, pollution to the environment is avoided, and the preparation process is simple, convenient and easy to implement and low in cost.

The invention discloses application of an inhibitor acting on NOS1 related ceRNA in relieving sheep endometritis caused by LPS and promoting embryo implantation, and relates to the field of bioengineering. The inhibitor is si-NOS1 (SEQ ID No.1) and / or si-TCONS00000014 (SEQ ID No.2), and by regulating and controlling a NOS1 / bta-miR-370 / TCONS00000014 signal axis, the endometrial oxidative stress excessive activation induced by LPS (lipopolysaccharide) is improved, the autophagy and apoptosis of cells are relieved, and the expression of a key implantation gene is recovered. Experiments prove that the inhibitor can significantly improve the sheep embryo implantation rate and reduce the embryo loss probability, provides a new target and strategy for preventing and treating LPS-induced sheep endometritis and embryo implantation disorder, and has important significance for improving the sheep breeding efficiency.

The invention provides application of uterus-derived sphingomonas DS-28 and a metagen thereof in treatment of endometritis, and relates to the technical field of microorganisms. The sphingomonas DS-28 provided by the invention has uterus colonization ability and good ecological niche adaptability, and also has a remarkable curative effect in treatment of Escherichia coli induced endometritis. Animal experiments show that the strain preparation can effectively relieve red and swollen uterus, relieve pathological damage, remarkably reduce gene expression of TNF-alpha, IL-6 and IL-8 and inhibit an NF-kappa B signal channel, and the anti-inflammatory and tissue repair effects of the strain preparation are superior to those of other sphingomonas strains C3-25.1, C3-41.1 and C3-42.1. Meanwhile, the metastatic preparation of the strain can also relieve mouse escherichia coli endometritis, improve red and swollen uterus and relieve pathological injury.

The invention belongs to the technical field of veterinary drug preparation, and discloses a synergistic doxycycline hydrochloride effervescent tablet as well as a preparation method and application thereof. The effervescent tablet is prepared from the following raw and auxiliary materials in percentage by weight: 10-15% of doxycycline hydrochloride, 3-5% of composite essential oil microcapsules, 3-7% of bletilla striata polysaccharide, 7-10% of Arabic gum, 1-3% of lauryl sodium sulfate, 38-42% of an effervescing agent, 5-6% of an adhesive, 4-5% of a disintegrating agent, 2-3% of a lubricant and the balance of a filling agent, wherein the weight percentage of the raw and auxiliary materials is 100%. The effervescent tablet prepared by the invention can be completely disintegrated after encountering water, can generate a large number of bubbles and is high in disintegration speed, the contact area between the medicine and an inflammation part is effectively enlarged, the microcapsules can be well adhered to the inner walls of the uterus and the vagina after absorbing water and expanding, the retention time of the medicine is prolonged, and the medicine effect is more lasting. The effervescent tablet is applied to treatment of bovine vaginitis and endometritis, the cure rate can reach 90% or above, and the effervescent tablet is suitable for being applied to intensive large-scale farms.

The invention belongs to the technical field of biological medicine, and particularly relates to application of chitosan in preparation of endometritis diagnostic products, objective quantitative detection is achieved through electric signal response of a chitosan memristor, accurate recognition of early and latent inflammations is achieved by means of high-sensitivity response of a chitosan material to low-concentration inflammatory factors, and the application of the chitosan material in preparation of endometritis diagnostic products is achieved. The selective binding capacity of a chitosan material to specific pathogen metabolites in local endometrium is utilized, so that the detected tissue specificity is enhanced, and cross interference is reduced.

The present invention relates to peptides, fusion proteins and compositions for inducing an immune response against bacteria. The invention also relates to the treatment or prevention of infections by sepsis-inducing bacteria, and to the treatment and prevention of pathological conditions caused by such bacteria. Such pathological conditions include sepsis, pneumonia, meningitis, endocarditis, enterocolitis, urinary tract infection, soft tissue infection, gastrointestinal tract infection, bloodstream infection, encephalitis, premature delivery and dead delivery.