The present invention discloses an instrument apparatus for low-temperature storage of a biological material. The instrument apparatus at least comprises a low-temperature protection agent storage tank, a protection agent dilution buffer liquid storage tank, a miniature electromagnetic valve, a miniature electromagnetic valve, a constant flow pump, a programmable controller, a PC computer, a data acquisition card, an electromagnetic feedback density sensing system, a temperature measurement sensor, sterile filters, a container cover, a biological material storage container, a stainless steel container housing, a temperature control device, a small peristaltic pump, and a valve. According to the present invention, with the instrument apparatus, the addition of the low-temperature protection agent can be strictly controlled, the whole cooling process can be controlled, the low-temperature protection agent is synchronously subjected to elution during the rewarming control, the toxicity of the low-temperature protection agent can be minimized, the tissue cells can achieve the low temperature state within the specified time range under the room temperature state, and the specimen can recover to the room temperature state from the low temperature state within the specified time if necessary.