The invention relates to a 3D printing personalized plunger type puncture treatment template with a fixing device. The treatment template comprises a template base plate, template guide holes, puncture needle guide columns, fine adjustment bracket insertion holes, positioning holes, lightweight through holes, laser alignment lines, puncture needle numbers, depth marks and the like. The template puncture needle guide columns can be taken down at any time, the diameter of the template guide holes is large and the length of the template guide holes is small after template puncture needle guide columns are taken down, so that local anesthesia points are accurate in positioning, local anesthesia is easy to implement, a bone drill with a large diameter can be conveniently used, and drilling andpositioning are precise. The three fine adjustment bracket insertion holes are formed and matched with a fine adjustment bracket in use so that the position of the template can be fine adjusted and fixed. The puncture needle numbers and the depth marks are arranged on the surface of the template base plate, and the situation that puncture needles are inserted by mistake because no depth marks arearranged is prevented. The template is customized, each puncture needle is precisely designed before operations according to CT, magnetic resonance and other medical images, precise minimally invasivesurgery is achieved, operation difficulty and operation risks are greatly reduced, the learning cycle of young doctors is shortened, services are quickly conducted, and then more patients are benefited.