The invention discloses a preparation method of glufosinate-ammonium -2, 4-drop soluble concentrate. The preparation method of the glufosinate-ammonium-2, 4-drop soluble concentrate comprises the following steps of: mixing 2, 4-drop active compound with a dimethylamine aqueous solution to generate 2, 4-drop dimethylamine salt, accurately weighing glufosinate-ammonium active compound, propylene glycol monomethyl ether, ethylene glycol and dodecyl ether sulfuric acid, adding water, mixing and stirring, and standing to obtain the glufosinate-ammonium 2, 4-drop soluble concentrate, wherein the mass ratio of the 2, 4-drop active compound to the dimethylamine to the glufosinate-ammonium to the propylene glycol monomethyl ether to the ethylene glycol to the dodecyl ether sulfuric acid to the water is (1.77-2.40): (1.02-1.05): (9.47-11.58): (0.75-0.85): (4.85-5.15): (3.8-4.2): (78.34-74.77). According to the invention, 2, 4-drops and glufosinate-ammonium are mixed according to a proper ratio to prepare glufosinate-ammonium 2, 4-drops soluble concentrate, so that the generation of herbicide resistance of weeds is delayed, the weeding range is expanded, the pesticide effect is improved, thedosage is reduced, the economic cost is saved, and the effective storage life of the glufosinate-ammonium 2, 4-drops soluble concentrate is more than two years.