The invention discloses a rat craniocerebral model preparation device which comprises a sliding block, a
drill rod, at least two limiting
insertion holes and limiting
insertion pieces, wherein the sliding block is arranged in a sliding mode and provided with a guide hole, the
drill rod is sleeved with a locking sleeve, the limiting
insertion holes are distributed in one side of the sliding block in the axial direction of the guide hole and communicated with the guide hole in the radial direction, and the limiting insertion piece is inserted into one limiting insertion hole. When the sliding block slides to a designated area, the limiting insertion piece is inserted into the limiting insertion hole in the uppermost layer, the
drill rod moves downwards along the guide hole until the drill rod abuts against the
skull, the locking sleeve which is not locked moves downwards along the drill rod to abut against the limiting insertion piece when the drill rod moves downwards along the guide hole to abut against the
skull, the locking sleeve is locked on the drill rod, and the limiting insertion piece is pulled out; when the limiting insertion pieces are sequentially inserted into the limiting insertion holes of the subsequent
layers from top to bottom, the locked locking sleeve drills into the
skull along with the drill rod till the locking sleeve abuts against the limiting insertion pieces again, the drilling depth of the drill rod is equal to the distance between all the limiting insertion holes, the drilling depth of the drill rod is accurately controlled, and the situation that the drill rod damages the brain is avoided.