5 results about "Epigenetic Change" patented technology

The invention discloses a sperm genome methylation detection method for safety evaluation of biological breeding crops by using primates and application, and relates to the technical field of safety evaluation of crops, the sperm genome methylation detection method comprises the following steps: dividing non-human primates into three groups, collecting sperms after long-term feeding, and extracting DNA (Deoxyribose Nucleic Acid); carrying out whole genome sequencing and quality control after bisulfite treatment; the epigenetic safety of crops is comprehensively evaluated by analyzing the methylation level of a whole genome and a functional region and functional enrichment of a differential methylation region and related genes thereof; according to the sperm genome methylation detection method for evaluating the safety of the biologically bred crops by utilizing the primates and the application, by utilizing a high-resolution WGBS technology, subtle epigenetic changes which are difficult to find by traditional toxicology can be detected, and the sperm genome methylation detection method has important significance in cross-generation reproduction effect evaluation, and has a wide application prospect. A food safety evaluation system can be perfected, and a more scientific and reliable safety interpretation basis can be established.

The present invention relates to a method for preparing a single cell multi-sample multi-body library and a use thereof. The present invention relates to a method whereby an epigenome and a transcriptome can be simultaneously analyzed at a single cell level, and the principle of regulating the epigenome and the transcriptome has been identified. Accordingly, the present invention makes it possible to reduce reagent costs by about 1 / 10 compared to the prior art and simultaneously analyze six or more samples, thus being economical and having the advantage of being able to contribute to high-tech genomic research. According to the present invention, six samples can be simultaneously analyzed, and ATAC as an epigenome and 5 prime RNA information as a transcript can be simultaneously collected and used to clearly identify the starting point of gene expression that immediately responds to epigenetic changes.

The invention relates to a multi-factor regulation and control system and method for accelerated breeding of plants. The system comprises a collection module, a processing module and a measurement module. Based on a comparison result generated by a functional gene sequence provided by the measurement module and a standard functional gene sequence stored in a database, the processing module generates an instruction for controlling the collection module to collect the plant image and provides environmental parameters conforming to the plant growth tendency for the regulation and control module. The method comprises the following steps: comparing plant functional gene sequences; determining an abnormal site of the functional gene sequence; when the abnormal site of the functional gene sequence is consistent or partially consistent with the abnormal site which is stored in the database and affects the expression of the functional gene, the processing module generates an instruction for controlling the collection module to collect the image of the first plant phenotype. By comparing the plant functional gene sequences, the abnormal sites of the functional gene sequences can be quickly and accurately identified, so that whether the plant has gene mutation or epigenetic change which affects the growth and development of the plant can be judged.

Provided herein is a cell comprising: (a) a polynucleotide encoding a binding-triggered transcriptional switch (BTTS) comprising an intracellular domain comprising a DNA binding domain (DBD) and one or more epigenetic editors and (b) a nucleic acid comprising a binding site for the DBD, a promoter and, operably linked to the promoter, a coding sequence encoding a payload. Binding of the BTTS to an antigen results in decreased expression of the payload via epigenetic modifications. If the payload is a transcriptional repressor, then binding of the BTTS results in increased expression of a second payload that is operably linked to the first payload. These changes result in long-term epigenetic changes in expression of the payloads and can be used to steer cell fate or function changes in response to BTTS detected inputs.

Methods and systems are provided for the genetic and epigenetic profiling of DNA samples and the detection of genetic and epigenetic changes in such samples. These methods involve DNA digestion with methylation-sensitive restriction enzymes, followed by high-throughput sequencing and analysis of the sequence reads. The methods and systems of the present invention are advantageous, sensitive, and accurate, and allow working with very small quantities of DNA, obtaining a large amount of information, including methylation data, mutation data, and more, from the sequencing data of a single run.