The invention discloses a method for observing the shape and number of synapses in a dentate gyrus region based on
image analysis, and relates to the technical field of neurobiology. The method comprises the following steps: S1, tissue treatment: after an
experimental animal is anesthetized, pouring a
buffer solution and a stationary solution into the heart in stages, separating
hippocampus tissue, trimming and fixing, pre-cooling,
slicing and preserving at low temperature; s2,
dyeing and marking: performing targeted marking on the dentate gyrus area of the sea horse by adopting DiI particles through a fine
tungsten steel through needle, and preserving at low temperature after incubation; s3, fixing and mounting: fixing for the second time, and mounting with an anti-
fluorescence quenching agent after
DAPI nuclear
dyeing; s4,
confocal imaging is carried out, exclusive parameter layer scanning is set, and qualified
dendritic spine images are screened; and image quantitative analysis can be further carried out, and accurate quantification is realized according to classification standards. The method is standard in operation, greatly improves
sample quality and experimental result credibility, optimizes experimental efficiency and
controllability and reduces technical threshold by means of targeted marking and standardized processes, and is suitable for relevant basis and application research of the hippocampal synapses.