56 results about "Trail pheromone" patented technology

A composition including a bait and a borate is used to mitigate a population of target insects and / or mollusca. The composition may include attractive by-products of agriculture and manufacturing with or without semiochemicals or biopesticides prepared as point source units (such as pellets or microcapsules). The by-products attract insects and / or mollusca to a food source for the purpose of mitigating the target organism. The bait is combined with borates and is pelletized and / or encapsulated into micron size particles. The pelletized and / or encapsulated particles are broadcast (spread) over areas infested with the target insect. The insects ingest the composition and regurgitate the composition to the remainder of the insect population. Significant mitigation of the population of the target insects results with little to no effect on the environment. The composition is also passed on to the next generation due to cannibalism of the target insects. A feed through process wherein the composition is fed to an animal and is contained in the manure of the animal deposited as a point source location. The composition is either mixed in with the food of the animal or placed directly in the gut of the animal using a ball and gun method. The target insects are attracted to the manure and upon ingesting the manure, ingest the composition and are dispatched.

The present invention relates generally to polymer devices impregnated with semiochemical attractants for termites. The materials for delivering the semiochemicals are preferably cellulose-free. Devices of the subject invention are placed in soil and provide a slow-release and permeation of the semiochemicals into the adjacent soil so that termites tunneling in the vicinity are directed toward the chemical sources. Because the semiochemicals are impregnated in the polymer, the device also protects the semiochemicals so that they do not degrade rapidly under field conditions. In a preferred embodiment, the subject invention comprises a durable station housing, preferably with a removable bait, wherein the body of the station housing itself is impregnated with a semiochemical attractant.

Provided herein are genetically modified microbes. In one embodiment, a genetically modified microbe includes an exogenous polynucleotide comprising a pheromone-responsive region. In one embodiment, the pheromone-responsive region is derived from a conjugative plasmid from a member of the genus Enterococcus spp. The pheromone-responsive region includes a pheromone-responsive promoter and an operably linked coding region encoding an antimicrobial peptide. In one embodiment, a genetically modified microbe includes an exogenous polynucleotide comprising a promoter and an operably linked coding sequence encoding an antimicrobial peptide, where expression of the coding region is controlled by a modulator polypeptide and is altered by a modulating agent, and where the coding region encodes an antimicrobial peptide. Also provided herein are methods of using the genetically modified microbes, including methods for inhibiting growth of an Enterococcus spp., a pathogenic E. coli, or a pathogenic Salmonella spp., for treating a subject, and for modifying a subject's gastrointestinal microflora.

The present invention makes available a rapid, reproducible, robust assay system for screening and identifying pharmaceutically effective compounds that specifically interact with and modulate the activity of a cellular protein, e.g., a receptor or ion channel. The subject assay enables rapid screening of large numbers of compounds to identify those which act as an agonist or antagonist to the bioactivity of the cellular protein. In this system, the cell is treated with a compound, and functional interaction of this compound with a cellular receptor yields a detectable signal, which can be specifically measured. The subject assays include methods of identifying compounds which specifically modulate, for example, heterologous receptors coupled to the pheromone response pathway in yeast. The subject assays are particularly amenable to the identification of specific agonists and antagonists of G protein-coupled receptors.

The invention provides a release-controlled preparation for simultaneously interfering pest mating and attracting beneficial insects. The release-controlled preparation comprises pheromone for interfering the pest mating and an attractant for attracting the beneficial insects. The release-controlled preparation has the advantages that the release-controlled preparation is released into the surrounding environments in a gas form, so as to interfere the mating of the target insects in the effective stage, and attract the beneficial insects, such as bees; more importantly, the release rate of thepheromone for interfering the mating and the release rate of the attractant for attracting the target insects are different, so as to ensure that the different volatiles can complete the respective functions in the same carrier at different stages.

The invention discloses an assembly sequence planning method. The method can be divided into three stages, wherein at the first stage, assembly information is collected and a fitness function is established; at the second stage, the assembly sequence is found through the chaotic mixing algorithm; at the third stage, the fitness value is calculated, the scales of species are dynamically renewed, and route pheromones are renewed. The chaotic algorithm is added on the basis of the ant genetic algorithm, the chaotic algorithm search is introduced after the ant algorithm search is conducted so that the local search capacity of the algorithms is enhanced, feasible solutions fond through the two algorithms serve as initial species of the genetic algorithm, and the overall search capacity of the genetic algorithm is guided. Due to the fact that the initial species are enriched, the overall search capacity of the genetic algorithm is enhanced, and the search space is enlarged.

The present invention discloses systems and methods for controlling arthropod populations. The systems include a polymeric substrate, a semiochemical that is reactive upon an adult-stage arthropod, and an insecticide that is toxic to an immature-stage arthropod. The semiochemical may be a sex pheromone that disrupts mating behavior of the adult-stage arthropod. The insecticide may be a per os insecticide that only affects the immature-stage arthropod. The arthropod to be controlled may be gypsy moths, in which case the semiochemical may be disparlure and the insecticide may be spinosad. Further disclosed are methods for preparing systems for use in controlling arthropod populations.

The invention discloses a sustained-release fly trap and a preparation method thereof. The sustained-release fly trap is made from paper, fabric, plastic, rubber and wood materials as carrier and a fry trapping agent attached to the carriers. The fry trapping agent is composed of the following components in weight percentages: 0.87-1.40% of insecticide, 0.08-0.12% of fly lure, 1.00-5.00% of honey, 5.00-15.00% of surfactant, and solvents in balance. The carrier is flat and shaped like animal, food or fruit cartoon figure. The preparation method comprises spray-coating the fry trapping agent to the surface of the carrier or mixing the fry trapping agent with plastic particles and thermo-forming. The sustained-release fly trap has the advantages of good fly killing effect, moist resistance, long-lasting action, no harm to human body, easy production, good decoration effect, and environmental friendliness.

The invention discloses a method for preparing a fall webworm sex pheromone slow-releasing agent. The method comprises the following steps of: using (9Z, 12Z, 15Z)-octadecatrienal, (3Z, 6Z, 9S, 10R)-9, 10-epoxy-3, 6-heneicosadiene and (3Z, 6Z, 19S, 10R)-9, 10-epoxy-1, 3, 6-heneicosatriene as active components of sex pheromone, adding a stabilizer and then adding to polybutadiene resin; stirring to be uniform and obtaining a mixed solution; adding isocyanate which is isometric to the mixed solution into the mixed solution, continuously stirring and obtaining a paste-type finished fall webworm sex pheromone slow-releasing agent. The fall webworm sex pheromone slow-releasing agent disclosed by the invention has the advantages of single and high-efficient attraction effect to fall webworms, low price, non-pollution, good stabilization, non-volatility and wide prospect of application.

Provided herein are methods of generating genetically modified microorganisms, e.g., genetically modified yeast strains, which comprise functional disruptions in one or more pheromone response genes and one or more sporulation genes, and genetically modified yeast cells, e.g., genetically modified diploid and haploid yeast cells, that lack sporulation capability and endogenous mating capability, produced thereby.

The present invention makes available a rapid, reproducible, robust assay system for screening and identifying pharmaceutically effective compounds that specifically interact with and modulate the activity of a cellular protein, e.g., a receptor. or ion channel. The subject assay enables rapid screening of large numbers of compounds to identify those which act as an agonist or antagonist to the bioactivity of the cellular protein. In this system, the first cell is treated with a compound, and functional interaction of this compound with a cellular receptor yields a secreted signal. A second cell, bearing a receptor for this secreted signal, makes use of an indicator gene in a signaling pathway coupled to this second receptor. The subject assays include methods of identifying compounds which specifically modulate, for example, heterologous receptors coupled to the pheromone response pathway in yeast. The subject assays are particularly amenable to the identification of specific agonists and antagonists of G protein-coupled receptors.

This application describes novel cells that respond to a pheromone comrising a heterologous nucleic acid encoding a reporter operably linked to a pheromone-responsive promoter. The cells may further comprises a heterologous nucleic acid encoding an RGS protein. Also described are methods involving the use of these cells for detecting the ability of a test sample to alter RGS protein-mediated reporter gene expression.

The invention provides an insecticidal composition. The composition comprises insect pheromone, thiacloprid and novaluron. Results of the embodiments of the invention show that after the insect pheromone, thiacloprid and novaluron are compounded, results of indoor efficacy tests show that the compounded insect pheromone, thiacloprid and novaluron achieve a synergistic effect of controlling insectpests. Results of field trials show that for target insects of which adults have a certain migratory flight abilities, particularly in aviation plant protection, the synergistic effect even exceeds 50%.

The present invention makes available a rapid, reproducible, robust assay system for screening and identifying pharmaceutically effective compounds that specifically interact with and modulate the activity of a cellular protein, e.g., a receptor or ion channel. The subject assay enables rapid screening of large numbers of compounds to identify those which act as an agonist or antagonist to the bioactivity of the cellular protein. In this system, the cell is treated with a compound, and functional interaction of this compound with a cellular receptor yields a detectable signal, which can be specifically measured. The subject assays include methods of identifying compounds which specifically modulate, for example, heterologous receptors coupled to the pheromone response pathway in yeast. The subject assays are particularly amenable to the identification of specific agonists and antagonists of G protein-coupled receptors.

The invention belongs to the field of organic synthesis, and particularly relates to a synthesis method for ectropis oblique sex pheromone [cis-6,7-epoxy-(z,z)-3,9-octadecadiene]. The ectropis oblique sex pheromone is obtained by oxosynthesizing (z,z,z)-3,6,9-octadecatriene in the presence of a catalyst, namely Keggin type tetrabutylammonium divacant lacunary silicotungsate, wherein the mole ratio of (z,z,z)-3,6,9-octadecatriene to the catalyst to an oxidant is (4.8-5.2):(0.006-0.010):(0.8-1.2), the oxidization reaction temperature is at 40-60DEG C, and the oxidization reaction time is 3-8h. according to the synthesis method for ectropis oblique sex pheromone, the middle purification step can be omitted by taking low-cost linolenate as a starting raw material, the environment pollution can be decreased by adopting the green epoxidation method of hydrogen peroxide, the cost can be lowered, the productivity is improved, and the synthesis method provides a new thought and a new method for the green synthesis of the sex pheromone.

The invention provides a method for analyzing insect semiochemicals by combining GC-MS and an electroantennography and belongs to the technical field of screening and determination of compounds. According to the method, screening of the insect semiochemicals and identification of sample material components can be synchronously performed, experiment steps are simplified, quick and efficient, the consistency of experimental data can be ensured, and higher economy and practicality are realized. The technical scheme is that the method comprises the following steps: cutting off tentacles of insectsalong base parts, removing small parts of top ends, then respectively fixing two ends of in vitro tentacles on a probe of the electroantennography by using a conductive adhesive, and enabling the tentacles to be connected with a microelectrode; feeding a sample into a gas chromatograph, and carrying out GC-MS and an electroantennography combined analysis; carrying out mass spectrometric detectionon peaks appearing at the same retention time of a GC-MS chromatogram and an electroantennography chromatogram, and identifying the insect semiochemicals with the activity by checking a standard spectral library with a standard mass spectrogram of the compounds. The method provided by the invention can be applied in a sensory mechanism of the insects to the semiochemicals, screening of active pheromone components or secondary plant substances and the like.

The invention provides a method for trapping diamond back moths, and belongs to the technical field of pest control. The method includes the step: placing sex pheromone into light with the wavelengthof 350-595nm to trap the diamond back moths. The sex pheromone comprises cis-11-hexadecenal, cis-11-hexadecene acetate and cis-11-hexadecene alcohol, and the mass ratio of the cis-11-hexadecenal, thecis-11-hexadecene acetate to the cis-11-hexadecene alcohol is (2.5-5):(5-9):(0.1-1.5). By the method, the trapping quantity of the diamond back moths is increased.

The invention discloses a sparse distribution type sex pheromone releaser which comprises a first cavity (1), a second cavity (2) and a liquid container (11), wherein the bottom of the first cavity (1) is connected with the bottom of the second cavity (2), the first cavity (1) can contain the second cavity (2) to form an outer cavity (4) which is filled with liquid, inner space of the second cavity (2) forms an inner cavity (3), two kinds of openings or notches which are formed alternately are formed in the lower end of the first cavity (1) and the lower end of the second cavity (2), the openings or notches, belonging to one kind, are communicated with the inner cavity (3) and outer space, sex pheromones in the inner cavity (3) can be released to the outer space through a large arc opening (8), and the openings or notches, belonging to the other kind, are communicated with the outer cavity (4) and the outer space. The releaser solves the problems that in traditional insect sex pheromone isotropic prevention, waste is caused, and sex pheromone prevention cost is high due to the fact that the sex pheromones are easily oxidized to lose efficacy, are uncontrollable and are released in an all-weather mode.

The invention discloses a group robot system based on an infrared simulation artificial pheromone. The system comprises a light-transmitting substrate, a plurality of group robots and a plurality of infrared transceiving repeaters, wherein the group robots can move on a glass substrate, each group robot is provided with an infrared transceiver, and each infrared transceiver comprises two symmetrical infrared receivers and a plurality of infrared transmitters arranged according to a plurality of directions; each infrared transceiving repeater comprises a conical outer shell, a conical inner shell, an infrared receiver and an infrared transmitter, wherein the conical inner shell is coaxially fixed in the conical outer shell, the infrared transmitter is arranged between the conical outer shell and the conical inner shell, the inner surface of the conical outer shell is an infrared reflecting surface; and the group robots utilize the infrared transceivers to communicate with one another and the infrared transceiving repeaters, and utilize an ant colony algorithm based on the virtual pheromone to move on the light-transmitting substrate. The local communication of the group robots of the invention is stable, the overall control is simple and convenient, excessive manual intervention is not needed, and the anti-interference capability is high.

Provided herein are genetically modified microbes. In one embodiment, a genetically modified microbe includes an exogenous polynucleotide comprising a pheromone-responsive region. In one embodiment, the pheromone-responsive region is derived from a conjugative plasmid from a member of the genus Enterococcus spp. The pheromone-responsive region includes a pheromone-responsive promoter and an operably linked coding region encoding an antimicrobial peptide. In one embodiment, a genetically modified microbe includes an exogenous polynucleotide comprising a promoter and an operably linked coding sequence encoding an antimicrobial peptide, where expression of the coding region is controlled by a modulator polypeptide and is altered by a modulating agent, and where the coding region encodes an antimicrobial peptide. Also provided herein are methods of using the genetically modified microbes, including methods for inhibiting growth of an Enterococcus spp., a pathogenic E. coli, or a pathogenic Salmonella spp., for treating a subject, and for modifying a subject's gastrointestinal microflora.

The invention relates to a glue-spraying type pest attractant. The attractant comprises the following components in parts by weight: 90-95 parts of pest sticking glue, 3-8 parts of insect pheromone, 0.1-3 parts of 3-phenylpropionaldehyde and 0.1-3 parts of eucalyptol. The insect pheromone is prepared from anisaldehyde, p-tolualdehyde, ethyl 3- piperidinecarboxylate, 4-anisaldehyde, citronellol andeugenol according to the mass ratio of (1-15):(1-15):(1-15):(1-5):(1-5):(1-5). According to the invention, the compounded 3-phenylpropionaldehyde and the eucalyptol are used as plant-source synergists and can improve the attracting effect of the insect pheromone by 20%-35%. Meanwhile, the oily glue is utilized as a release agent and is sprayed on appliance surfaces, and when a diluent is volatilized, the high binding character of the colloid is utilized for high efficiently attracting and killing pests. The pest attractant provided by the invention meets the urgent requirements of glue-spraying type pest attractants with no public nuisance, high efficient attraction, good stickiness, high strength, good stability, simple and convenient operation and low cost on the market, and has a widemarket prospect.

The invention discloses a sticky insect board. The fluorescent plate emits light at night, because the pheromone coating and the sticky sticky film are both transparent materials, which will not affect the scattering of light, and can effectively attract phototaxis pests to fly to the sticky sticky film. , stuck and struggled to death. The pheromone coating emits odor, which spreads into the air through the air holes on the mucus membrane, which can effectively attract corresponding pests to fly to the mucilage membrane, where they struggle to death after sticking. In the process of placement, there is a handle board for taking, which can avoid sticking to the hand when transferring or handling, and the sticky insect area will lose its effect due to finger touch.

Provided are a behavior-disrupting agent of Argentine ants for controlling them or suppressing their reproduction is a safer and more effective manner, and the behavior-disrupting method of them. More specifically, a behavior-disrupting agent of Argentine ants comprising Z-9-hexadecenal, and a behavior-disrupting method of Argentine ants comprising a step of using the behavior-disrupting agent are provided based on the finding that the behavior of Argentine ants is disrupted by emission of Z-9-hexadecenal, which is a trail pheromone, into the atmosphere.

Nematode dispersal is one of the key features for success as a biocontrol agent. Currently, commercially available nematodes do not disperse sufficiently when they are applied to a field. Since the insect target is mobile, nematodes need to be actively moving and seeking an insect host. We developed a pheromone extract from nematode growth medium that disperses nematodes. This extract was unstable in liquid form. We have found that the extract can be dried to retain activity during storage or shipment. Exposing nematodes to pheromone extract before they are applied to a field activates them to disperse and seek a new host. This exposure needs to be at least 20 min. When nematodes are actively seeking a new host this increases nematode insect encounter and increases insect mortality leading to increased effectiveness of insect nematodes as biological control agents.

Provided herein are methods of generating genetically modified microorganisms, e.g., genetically modified yeast strains, which comprise functional disruptions in one or more pheromone response genes and one or more sporulation genes, and genetically modified yeast cells, e.g., genetically modified diploid and haploid yeast cells, that lack sporulation capability and endogenous mating capability, produced thereby.

An insect control substance for controlling certain flying insect pests in proximity to the substance includes a rubber crumb carrier having a plurality of rubber particles and a volatile hydrophobic semiochemical for attracting certain flying insect pests absorbed within the rubber particles forming a discontinuous phase of the substance. The insect control substance also includes a body of water forming a continuous phase of the substance within which the discontinuous phase is suspended. When the insect control substance is applied to an application surface the discontinuous phase is exposed to the surrounding air and the semiochemical within the rubber particles evaporates into the air around the application surface and attracts the flying insect pests to the rubber crumb carrier to facilitate control of the flying insect pests.

The invention discloses a halogenated pheromone analogue for controlling a spotted cutworm. The general formula of the halogenated pheromone analogue is ROCOCnHaXb. The halogenated pheromone analogue is capable of more effectively trapping and killing a male worm of the spotted cutworm and interfering with orientation of the male worm to a female worm, so that the encountering probability of the female worm and the male worm is reduced, mating is disturbed, the reproductive rate is reduced and extinction of pest populations is gradually controlled, thereby achieving the target of controlling injurious insects. The halogenated pheromone analogue is low in dosage and friendly to environment, can be accurately applied to insect condition prediction, mating disturbance and mass trapping, and is of great significance in comprehensive treatment of the spotted cutworm.

The invention discloses a sex pheromone releaser for insects. The sex pheromone releaser for insects comprises a shell and a container for containing sex pheromone, wherein the container is arranged in the shell. The sex pheromone releaser for insects is characterized in that one end of the container is provided with a nozzle; a driving mechanism and a control module for controlling the driving mechanism are arranged in the shell; the driving mechanism comprises a pressing part which can approach and press the nozzle or depart from the nozzle; and the shell is provided with a spraying port in the position corresponding to the nozzle. By arranging the pressing-type container and the driving mechanism capable of pressing the container in a controllable way, the container can be controlled to quantitatively spray the sex pheromones at set time so as to interfere with the mating of insects more stably and specifically, thereby avoiding the reproduction of the insects and achieving the goal of insect control. The slow releaser arranged outside the shell can slowly release the sex pheromones into air, thereby prolonging the release and action time of the sex pheromones, and further saving the consumption of the sex pheromones.

The invention discloses a three-spotted plusia sex attractant composite and application thereof, and belongs to the technical field of insect sex pheromone preparation. The sex attractant composite comprises three-spotted plusia sex pheromone and synergists. Wherein the components of the sex pheromone are cis-7-dodecene acetate, cis-11-hexadecane acetate and cis-11-hexadecane aldehyde; the synergists are mainly one or more of plant volatile 2-ethyl-1-hexanol, ar-turmerone and pentanal; mass ratio of the sex pheromone to the synergists is 1:0.3-1.2. The composite has the advantages of simple preparation and convenient use, the composite can be accurately used for the insect pest monitoring, massive trapping and mating interference of the three-spotted plusia, and the composite has significant meaning on comprehensively treating the three-spotted plusia.