The invention provides a method for analyzing
insect semiochemicals by combining GC-MS and an electroantennography and belongs to the technical field of screening and determination of compounds. According to the method, screening of the
insect semiochemicals and identification of sample material components can be synchronously performed, experiment steps are simplified, quick and efficient, the consistency of experimental data can be ensured, and higher economy and practicality are realized. The technical scheme is that the method comprises the following steps:
cutting off tentacles of insectsalong base parts, removing small parts of top ends, then respectively fixing two ends of
in vitro tentacles on a probe of the electroantennography by using a conductive
adhesive, and enabling the tentacles to be connected with a
microelectrode; feeding a sample into a gas chromatograph, and carrying out GC-MS and an electroantennography combined analysis; carrying out
mass spectrometric detectionon peaks appearing at the same
retention time of a GC-MS chromatogram and an electroantennography chromatogram, and identifying the
insect semiochemicals with the activity by checking a standard spectral
library with a standard
mass spectrogram of the compounds. The method provided by the invention can be applied in a sensory mechanism of the insects to the semiochemicals, screening of active
pheromone components or secondary
plant substances and the like.