The present invention discloses a vehicle-borne display device comprehensive testing device which comprises a testing master control machine for managing a test case and analyzing a testing result, a man-machine interface testing module which is connected to the testing master control machine, simulates a man-machine interaction screen touch operation according to the test case, obtains the output image of a tested display device, and analyzes the man-machine interaction display function and reliability of the tested display device, a communication interface testing module which is connected to the testing master control machine, monitors the operation state of the tested display device and analyzes the communication reliability and stability of the tested display device, and the tested display device which is connected to the man-machine interface testing module and the communication interface testing module. The invention also discloses a testing method, according to testing requirements, a code white box test, a man-machine interaction test and a communication interaction test are executed, the testing result data is analyzed, and a testing report is outputted. Complex communication and electrical environments can be simulated, the degree of automation is high, and the test coverage is wide.