The invention relates to a disinfection device, in particular to a rotatable medical instrument disinfection device. The device comprises a box cover, a disinfectant box, a device shell, a disinfectant collection box and a device inner box, the box cover is in threaded connection with the disinfectant box, the disinfectant box is mounted on the device shell, the disinfectant collection box is mounted on the device shell, the device inner box is fixedly installed in the device shell, a door is mounted on the device shell, a door is mounted on the device inner box, a pressure device is arrangedon the disinfectant box, an adjustable spraying mechanism is mounted on the device shell, the adjustable spraying mechanism is mounted on the device inner box in a sliding manner, an instrument uniformly-placing mechanism is mounted on the device inner box, and an instrument fixing mechanism is mounted on the instrument uniformly-placing mechanism. According to the device, instruments can be automatically and evenly placed in a separated mode, manual operation is not needed, the device can reverse the instruments, multi-face disinfection of the instruments is achieved, the distance between a disinfectant spraying opening and the instruments can be freely adjusted through the device, the spraying effect is better, and the disinfection effect is better.