A novel quantitative pesticide taking device comprises a bottom plate, a frame device, a cover plate device, a retaining plate device and a rotating device. A first support, a second support, a first supporting rod and a first transverse rod are arranged on the bottom plate. The frame device comprises a frame, third supports, a blocking plate, a first filter screen, a transverse plate, a pipeline and a valve. The cover plate device comprises a first vertical rod, a first oblique rod, a second transverse rod, a first spring, a cover plate and a holding ring. The retaining plate device comprises a second vertical rod, a moving plate, a seal plate, a limiting block and a second spring. The rotating device comprises an abutment plate, a fourth support, a fifth support, a rotating rod, a first fixing rod, a third spring, a collection frame and a positioning plate. By the aid of the novel quantitative pesticide taking device, a certain quantity of pesticides can be prepared, pesticide concentration can be determined, and accordingly pesticidal effects of the pesticides are guaranteed, and pesticide waste is reduced.