The invention relates to a plastic bottle negative pressure detecting device which comprises a base, a cavity, a water container, a fixing unit and a pneumatic device. An air inlet hole is formed in the base, the cavity is formed in the base, a pressure applying cavity is formed by the cavity and the base, the air inlet hole is communicated with the pressure applying cavity, the water container is arranged above the cavity, the fixing unit is arranged between the cavity and the water container, and the pneumatic device is communicated with the air inlet hole. According to the plastic bottle negative pressure detecting device, pressure is applied to the exterior of a bottle opening through a negative pressure device, compressed air enters the pressure applying cavity through a reducing valve to apply pressure according to requirements, a tested sample has the internal-external differential pressure, the air overflowing conditions of the tested sample are observed, and the sealing performance of the tested sample is accordingly judged.