The present invention relates to a prefabricated building prefabricated steel structure connection and positioning machine and construction method, comprising an L-shaped fixing seat, an electro-hydraulic push rod, a reinforcing rod, a connecting plate, a supporting seat, a placing seat, a clamping mechanism and a driving mechanism. The number of L-shaped fixed seats is two, one end of the two L-shaped fixed seats is fixed by a connecting plate, one side of the L-shaped fixed seat is provided with a reinforcing rod, and the upper end of the L-shaped fixed seat is equipped with an electro-hydraulic push rod, The outer wall of the electrohydraulic push rod is welded with a support tray, the upper end of the support tray is installed with a support seat, the top center of the support seat is installed with a drive mechanism, the top of the electrohydraulic push rod is welded with a placement seat, and both sides of the placement seat are installed With the clamping mechanism, the invention can complete the connection and positioning of two steel beams at the same time, with high work efficiency, and ensures the same feed rate of the two steel beams. At the same time, the multi-stage clamping mechanism is used to fix the steel beams to ensure clamping There will be no slippage at the contact surface between the structure and the steel beam, and repeated operations are not required.